Anti-Neuraminidase NEU2 Antibody

Product Name

Anti-Neuraminidase NEU2 Antibody

View all NEU2 Antibodies

SKU/Catalog Number

A06481

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Neuraminidase NEU2 Antibody (Catalog # A06481). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Neuraminidase NEU2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06481)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion near aa 100-125 of Human Neu2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06481 is reactive to NEU2 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

42254 MW

Background of NEU2

Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of α-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-α-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library. The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06481 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: User optimized
IP: User optimized
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For NEU2 (Source: Uniprot.org, NCBI)

Alternative Names

Neu2, neuraminidase 2, Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase-2, NEU-2, Neu 2 NEU2 SIAL2 neuraminidase 2 sialidase-2|N-acetyl-alpha-neuraminidase 2|cytosolic sialidase|sialidase 2 (cytosolic sialidase)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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