Anti-Netrin 1/NTN1 Antibody Picoband®

Netrin-1 antibody

Boster Bio Anti-Netrin 1/NTN1 Antibody catalog # PA1662. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 2 publication(s).

Product Info Summary

SKU: PA1662
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Netrin 1/NTN1 Antibody Picoband®

View all Netrin-1 Antibodies

SKU/Catalog Number

PA1662

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Netrin 1/NTN1 Antibody catalog # PA1662. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Netrin 1/NTN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1662)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1662 is reactive to NTN1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

8 kDa

Calculated molecular weight

67748 MW

Background of Netrin-1

Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1662 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: U87 Cell, COLO320 Cell,

Validation Images & Assay Conditions

Gene/Protein Information For NTN1 (Source: Uniprot.org, NCBI)

Gene Name

NTN1

Full Name

Netrin-1

Weight

67748 MW

Alternative Names

Netrin-1;Epididymis tissue protein Li 131P;NTN1;NTN1L; NTN1 MRMV4L, NTN1 netrin 1 netrin-1|epididymis tissue protein Li 131P|netrin 1, mouse, homolog of

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NTN1, check out the NTN1 Infographic

NTN1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NTN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1662 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhong S, Zou L, Zhao Y, Hu B, Xie H. J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):500-4. Doi: 10.1007/S11596-010-0457-X. Epub 2010 Aug 17. Effect Of Different Concentrations Of Neogenin On Proliferation, Apoptosis And Related Proliferativ...

Qian-Hua W, Shao-Ping Z, Jian-Wen Z, Yun Y, Li Z. Mol Cell Biochem. 2011 Apr;350(1-2):81-7. Doi: 10.1007/S11010-010-0684-2. Epub 2010 Dec 31. Reduced Expression Of Netrin-1 Is Associated With Fetal Growth Restriction.

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3 Customer Q&As for Anti-Netrin 1/NTN1 Antibody Picoband®

Question

I have a question about product PA1662, anti-Netrin 1/NTN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1662 anti-Netrin 1/NTN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-11

Question

Does PA1662 anti-Netrin 1/NTN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-06-27

Answer

It shows on the product datasheet, PA1662 anti-Netrin 1/NTN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-27

Question

We are currently using anti-Netrin 1/NTN1 antibody PA1662 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

The anti-Netrin 1/NTN1 antibody (PA1662) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-24

Order DetailsPrice
PA1662

100μg

$370
PA1662-10ug

10μg sample (liquid)

$99
PA1662-Biotin

100 μg Biotin conjugated

$570
PA1662-Cy3

100 μg Cy3 conjugated

$570
PA1662-Dylight488

100 μg Dylight488 conjugated

$570
PA1662-Dylight550

100 μg Dylight550 conjugated

$570
PA1662-Dylight594

100 μg Dylight594 conjugated

$570
PA1662-FITC

100 μg FITC conjugated

$570
PA1662-HRP

100 μg HRP conjugated

$570
PA1662-APC

100 μg APC conjugated

$670
PA1662-PE

100 μg PE conjugated

$670
PA1662-iFluor647

100 μg iFluor647 conjugated

$670
PA1662-carrier-free

Carrier Free

$370

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PA1662
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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