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Product Info Summary
| SKU: | A10929-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NEK11 Antibody Picoband®
SKU/Catalog Number
A10929-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NEK11 Antibody Picoband® catalog # A10929-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NEK11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10929-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NEK11 recombinant protein (Position: E72-Q630). Human NEK11 shares 71.1% amino acid (aa) sequence identity with mouse NEK11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10929-2 is reactive to NEK11 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
74 kDa
Calculated molecular weight
74.192kDa
Background of NEK11
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10929-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human Hela whole cell lysates, human HepG2 whole cell lysates, human A431 whole cell lysates, human A549 whole cell lysates, rat testis tissue lysates, rat C6 whole cell lysates, mouse testis tissue lysates, mouse Neuro-2a whole cell lysates
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NEK11 using anti-NEK11 antibody (A10929-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK11 antigen affinity purified polyclonal antibody (Catalog # A10929-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEK11 at approximately 74 kDa. The expected band size for NEK11 is at 74 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NEK11 (Source: Uniprot.org, NCBI)
Gene Name
NEK11
Full Name
Serine/threonine-protein kinase Nek11
Weight
74.192kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; FLJ23495; Never in mitosis A-related kinase 11; NIMA (never in mitosis gene a)- related kinase 11; NimA-related protein kinase 11; serine/threonine-protein kinase Nek11 NEK11 NIMA related kinase 11 serine/threonine-protein kinase Nek11|NIMA (never in mitosis gene a)- related kinase 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEK11, check out the NEK11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEK11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEK11 Antibody Picoband® (A10929-2)
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