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Product Info Summary
| SKU: | A11525 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-NECAB3/Apba2Bp Antibody
SKU/Catalog Number
A11525
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NECAB3/Apba2Bp Antibody (Catalog # A11525). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NECAB3/Apba2Bp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11525)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NECAB3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11525 is reactive to NECAB3 in Human, Mouse
Reconstitution
Calculated molecular weight
44350 MW
Background of Necab3
Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta-amyloid.
Sugita S., Neuroscience 112:51-63(2002).
Yoo J.C., Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11525 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, HepG2 cells and HT-29 cells, using NECAB3 antibody A11525.
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Immunofluorescence analysis of A549 cells, using NECAB3 antibody A11525.
Protein Target Info & Infographic
Gene/Protein Information For NECAB3 (Source: Uniprot.org, NCBI)
Gene Name
NECAB3
Full Name
N-terminal EF-hand calcium-binding protein 3
Weight
44350 MW
Alternative Names
N-terminal EF-hand calcium-binding protein 3;Amyloid beta A4 protein-binding family A member 2-binding protein;Nek2-interacting protein 1;Neuronal calcium-binding protein 3;X11L-binding protein 51;NECAB3;APBA2BP, NIP1, SYTIP2, XB51;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NECAB3, check out the NECAB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NECAB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NECAB3/Apba2Bp Antibody (A11525)
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No publications found for A11525
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Customer Q&As
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3 Customer Q&As for Anti-NECAB3/Apba2Bp Antibody
Question
We are currently using anti-NECAB3/Apba2Bp antibody A11525 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The anti-NECAB3/Apba2Bp antibody (A11525) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
I was wanting to use your anti-NECAB3/Apba2Bp antibody for IF for human placenta skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta skeletal muscle identification?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
You can see on the product datasheet, A11525 anti-NECAB3/Apba2Bp antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human placenta skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-08
Question
Is this A11525 anti-NECAB3/Apba2Bp antibody reactive to the isotypes of NECAB3?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The immunogen of A11525 anti-NECAB3/Apba2Bp antibody is Synthesized peptide derived from internal of human NECAB3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-29
