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Product Info Summary
| SKU: | A08623 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NDUFB9 Antibody
SKU/Catalog Number
A08623
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NDUFB9 Antibody catalog # A08623. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDUFB9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08623)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NDUFB9. AA range:102-151
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08623 is reactive to NDUFB9 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
21831 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08623 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of COLO205 cells using NDUFB9 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using NDUFB9 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NDUFB9 (Source: Uniprot.org, NCBI)
Gene Name
NDUFB9
Full Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
Weight
21831 MW
Superfamily
complex I LYR family
Alternative Names
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9;Complex I-B22;CI-B22;LYR motif-containing protein 3;NADH-ubiquinone oxidoreductase B22 subunit;NDUFB9;LYRM3, UQOR22; NDUFB9 B22, CI-B22, LYRM3, MC1DN24, UQOR22 NADH:ubiquinone oxidoreductase subunit B9 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9|LYR motif-containing protein 3|NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa|NADH-ubiquinone oxidoreductase B22 subunit|complex I B22 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFB9, check out the NDUFB9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFB9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFB9 Antibody (A08623)
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No publications found for A08623
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-NDUFB9 Antibody
Question
Would A08623 anti-NDUFB9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
It shows on the product datasheet, A08623 anti-NDUFB9 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-12
Question
I was wanting to use your anti-NDUFB9 antibody for WB for human cerebellum on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cerebellum identification?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
As indicated on the product datasheet, A08623 anti-NDUFB9 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
We are currently using anti-NDUFB9 antibody A08623 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The anti-NDUFB9 antibody (A08623) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-08
Question
We need to test anti-NDUFB9 antibody A08623 on human cerebellum for research purposes, then I may be interested in using anti-NDUFB9 antibody A08623 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The products we sell, including anti-NDUFB9 antibody A08623, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-29
Question
Does anti-NDUFB9 antibody A08623 work for WB with cerebellum?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
According to the expression profile of cerebellum, NDUFB9 is highly expressed in cerebellum. So, it is likely that anti-NDUFB9 antibody A08623 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2019-05-22
Question
I see that the anti-NDUFB9 antibody A08623 works with WB, what is the protocol used to produce the result images on the product page?
P. Roberts
Verified customer
Asked: 2014-06-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-06-09
