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Product Info Summary
| SKU: | A30499 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-NDF2 Antibody
SKU/Catalog Number
A30499
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NDF2 Antibody (Catalog# A30499). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30499)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NDF2. Immunogen sequence location: 61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30499 is reactive to NEUROD2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
41361 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30499 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from 293, COLO, and Jurkat cells, using NDF2 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For NEUROD2 (Source: Uniprot.org, NCBI)
Gene Name
NEUROD2
Full Name
Neurogenic differentiation factor 2
Weight
41361 MW
Alternative Names
NEUROD2;BHLHA1;NDRF;Neurogenic differentiation factor 2;NeuroD2;Class A basic helix-loop-helix protein 1;bHLHa1;NeuroD-related factor;NDRF NEUROD2 DEE72, EIEE72, NDRF, bHLHa1 neuronal differentiation 2 neurogenic differentiation factor 2|class A basic helix-loop-helix protein 1|neuroD-related factor|neurogenic basic-helix-loop-helix protein|neurogenic differentiation 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEUROD2, check out the NEUROD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEUROD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDF2 Antibody (A30499)
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No publications found for A30499
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Customer Q&As
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4 Customer Q&As for Anti-NDF2 Antibody
Question
I am interested in to test anti-NDF2 antibody A30499 on human brain for research purposes, then I may be interested in using anti-NDF2 antibody A30499 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The products we sell, including anti-NDF2 antibody A30499, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-25
Question
I see that the anti-NDF2 antibody A30499 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-18
Question
My question regarding product A30499, anti-NDF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30499 anti-NDF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-14
Question
We are currently using anti-NDF2 antibody A30499 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
B. Dhar
Verified customer
Asked: 2016-11-14
Answer
The anti-NDF2 antibody (A30499) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-14
