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Product Info Summary
| SKU: | M03215 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Myosin Light Chain 2 Rabbit Monoclonal Antibody
View all Myosin Light Chain 2 Antibodies
SKU/Catalog Number
M03215
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myosin Light Chain 2 Rabbit Monoclonal Antibody catalog # M03215. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myosin Light Chain 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03215)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FHO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myosin Light Chain 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03215 is reactive to MYL2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
18789 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03215 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
IP 1:50
Positive Control
IHC: human heart tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human heart, using Myosin Light Chain 2 Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Myosin Light Chain 2 expression in Mouse heart lysate.
Protein Target Info & Infographic
Gene/Protein Information For MYL2 (Source: Uniprot.org, NCBI)
Gene Name
MYL2
Full Name
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Weight
18789 MW
Alternative Names
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform ;MLC-2 ;MLC-2v;Cardiac myosin light chain 2 ;Myosin light chain 2, slow skeletal/ventricular muscle isoform ;MLC-2s/v ;Ventricular myosin light chain 2 ;MYL2 ;MLC2 ; MYL2 CMH10, MLC-2s/v, MLC2 myosin light chain 2 myosin regulatory light chain 2, ventricular/cardiac muscle isoform|MLC-2|MLC-2v|RLC of myosin|cardiac myosin light chain 2|cardiac ventricular myosin light chain 2|myosin, light chain 2, regulatory, cardiac, slow|myosin, light polypeptide 2, regulatory, cardiac, slow|regulatory light chain of myosin|slow cardiac myosin regulatory light chain 2|truncated myosin light chain 2|ventricular myosin light chain 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYL2, check out the MYL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myosin Light Chain 2 Rabbit Monoclonal Antibody (M03215)
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No publications found for M03215
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Myosin Light Chain 2 Rabbit Monoclonal Antibody
Question
Is this M03215 anti-Myosin Light Chain 2 Rabbit Monoclonal antibody reactive to the isotypes of MYL2?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The immunogen of M03215 anti-Myosin Light Chain 2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Myosin Light Chain 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-12
Question
Is a blocking peptide available for product anti-Myosin Light Chain 2 Rabbit Monoclonal antibody (M03215)?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We do provide the blocking peptide for product anti-Myosin Light Chain 2 Rabbit Monoclonal antibody (M03215). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-26
Question
We are currently using anti-Myosin Light Chain 2 Rabbit Monoclonal antibody M03215 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
The anti-Myosin Light Chain 2 Rabbit Monoclonal antibody (M03215) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-08
