Product Info Summary
SKU: | A08448 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Myosin 1G MYO1G Antibody
SKU/Catalog Number
A08448
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Myosin 1G MYO1G Antibody (Catalog # A08448). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Myosin 1G MYO1G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08448)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human Myosin 1G protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08448 is reactive to MYO1G in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
116442 MW
Background of MYO1G
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. In general, myosins are protein complexes consisting of one or more myosin heavy chains, associated light chains and other proteins. Myosins function as molecular motors and use the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments. Myosins have magnesium-ATPase activity and bind actin. Myosins can be divided into classes that are distinguished based on sequence features of the motor, or head domain, but also have distinct tail regions that are believed to bind specific cargoes. Unconventional myosins exist. Myosin 1G is an unconventional myosin that is restricted to hematopoietic cells. Unconventional myosins are also critical for motility in amoeba and a mammalian paralog (Myo1C) is critical as a glucose transporter that recycles glucose in response to insulin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08448 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:40,000
IP: 1:100
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Myosin 1G expression in Jurkat whole cell lysates (lane 1), peripheral blood T cells lysates (lane 2), human spleen extract (lane 3), 300.19 (lane 4) and 293 cells, appear negative for Myosin 1G (lane 5). Myosin 1G at 70KD was detected using rabbit anti-FBXL4 Antigen Affinity purified polyclonal antibody (Catalog # ) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For MYO1G (Source: Uniprot.org, NCBI)
Gene Name
MYO1G
Full Name
Unconventional myosin-Ig
Weight
116442 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Myosin 1G, Unconventional myosin-Ig, Minor histocompatibility antigen HA-2, mHag HA-2, MYO1G MYO1G HA2, HLA-HA2, MHAG myosin IG unconventional myosin-Ig|minor histocompatibility HA-2|myosin-Ig
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYO1G, check out the MYO1G Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYO1G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myosin 1G MYO1G Antibody (A08448)
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No publications found for A08448
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Customer Q&As
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6 Customer Q&As for Anti-Myosin 1G MYO1G Antibody
Question
Is this A08448 anti-Myosin 1G antibody reactive to the isotypes of MYO1G?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The immunogen of A08448 anti-Myosin 1G antibody is A synthetic peptide corresponding to an internal region of human Myosin 1G protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-Myosin 1G antibody A08448 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The anti-Myosin 1G antibody (A08448) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-31
Question
Does anti-Myosin 1G antibody A08448 work for WB with b-cell?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
According to the expression profile of b-cell, MYO1G is highly expressed in b-cell. So, it is likely that anti-Myosin 1G antibody A08448 will work for WB with b-cell.
Boster Scientific Support
Answered: 2019-09-19
Question
Here is the WB image, lot number and protocol we used for b-cell using anti-Myosin 1G antibody A08448. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
I see that the anti-Myosin 1G antibody A08448 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-24
Question
Does anti-Myosin 1G antibody A08448 work on feline WB with spleen?
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
Our lab technicians have not validated anti-Myosin 1G antibody A08448 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Myosin 1G antibody A08448 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-10