Product Info Summary
SKU: | A32480 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MYORG Antibody Picoband™
SKU/Catalog Number
A32480
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MYORG Antibody Picoband™ catalog # A32480. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MYORG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32480)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MYORG recombinant protein (Position: Q207-R611). Human MYORG shares 92.9% amino acid (aa) sequence identity with mouse MYORG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32480 is reactive to MYORG in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
Background of MYORG
MYORG promotes myogenesis by activating AKT signaling through the maturation and secretion of IGF2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32480 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MYORG using anti-MYORG antibody (A32480).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse ovary tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYORG antigen affinity purified polyclonal antibody (Catalog # A32480) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYORG at approximately 87 kDa. The expected band size for MYORG is at 81 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-MYORG antibody (A32480).
Overlay histogram showing HepG2 cells stained with A32480 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYORG Antibody (A32480, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MYORG (Source: Uniprot.org, NCBI)
Gene Name
MYORG
Full Name
Myogenesis-regulating glycosidase
Weight
Superfamily
glycosyl hydrolase 31 family
Alternative Names
Myogenesis-regulating glycosidase MYORG IBGC7, KIAA1161, NET37 myogenesis regulating glycosidase (putative) myogenesis-regulating glycosidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYORG, check out the MYORG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYORG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYORG Antibody Picoband™ (A32480)
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