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Product Info Summary
| SKU: | M03238 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Dog, Human, Mouse, Pig, Rat, Cat |
| Host: | Mouse |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody
SKU/Catalog Number
M03238
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (Catalog # M03238). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Dog, Cat, Pig.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03238)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: F5D
Isotype
IgG1, kappa
Immunogen
Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03238 is reactive to MYOG in Dog, Human, Mouse, Pig, Rat, Cat
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
25037 MW
Background of Myogenin
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03238 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Anti-Myogenin Mouse Monoclonal Antibody (F5D)
Protein Target Info & Infographic
Gene/Protein Information For MYOG (Source: Uniprot.org, NCBI)
Gene Name
MYOG
Full Name
Myogenin
Weight
25037 MW
Alternative Names
Myogenin;Class C basic helix-loop-helix protein 3;bHLHc3;Myogenic factor 4;Myf-4;MYOG;BHLHC3, MYF4; MYOG MYF4, bHLHc3, myf-4 myogenin myogenin|class C basic helix-loop-helix protein 3|myogenic factor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYOG, check out the MYOG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYOG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (M03238)
Hello CJ!
M03238 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system
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