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Product Info Summary
| SKU: | A08496-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MYL4 Antibody Picoband®
SKU/Catalog Number
A08496-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MYL4 Antibody Picoband® catalog # A08496-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MYL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08496-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequencein the middle region of human MYL4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08496-2 is reactive to MYL4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
21.565kDa
Background of MYL4
Atrial Light Chain-1 (ALC-1), also known as Essential Light Chain, Atrial is a protein that in humans is encoded by the MYL4 gene. Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08496-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Positive Control
WB: mouse heart tissue lysates, human K562 whole cell lysates, rat heart tissue lysates
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MYL4 using anti-MYL4 antibody (A08496-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL4 antigen affinity purified polyclonal antibody (Catalog # A08496-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL4 at approximately 24 kDa. The expected band size for MYL4 is at 22 kDa.
Click image to see more details
Figure 2. Western blot analysis of MYL4 using anti-MYL4 antibody (A08496-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL4 antigen affinity purified polyclonal antibody (Catalog # A08496-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL4 at approximately 24 kDa. The expected band size for MYL4 is at 22 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MYL4 (Source: Uniprot.org, NCBI)
Gene Name
MYL4
Full Name
Myosin light chain 4
Weight
21.565kDa
Alternative Names
ALC1; AMLC; GT1; MLC1; Myosin light chain 1, embryonic muscle/atrial isoform; myosin light chain 4; Myosin light chain alkali GT-1 isoform; myosin, atrial/fetal muscle, light chain; myosin, light chain 4, alkali; atrial, embryonic; myosin, light polypeptide 4, alkali; atrial, embryonic; PRO1957 MYL4 ALC1, AMLC, GT1, PRO1957 myosin light chain 4 myosin light chain 4|atrial myosin light chain 1|myosin, atrial/fetal muscle, light chain|myosin, light chain 4, alkali; atrial, embryonic|myosin, light polypeptide 4, alkali; atrial, embryonic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYL4, check out the MYL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYL4 Antibody Picoband® (A08496-2)
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