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Product Info Summary
| SKU: | A04528 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MYH14/Myosin Heavy Chain Antibody
SKU/Catalog Number
A04528
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MYH14/Myosin Heavy Chain Antibody (Catalog # A04528). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MYH14/Myosin Heavy Chain Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04528)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MYH14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04528 is reactive to MYH14 in Human
Reconstitution
Calculated molecular weight
227871 MW
Background of Myh14
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping By similarity.
Leal A., Gene 312:165-171(2003).
Bienvenut W.V., Submitted (NOV-2006) to UniProtKB.
Nagase T., Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04528 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using MYH14 antibody A04528.
Protein Target Info & Infographic
Gene/Protein Information For MYH14 (Source: Uniprot.org, NCBI)
Gene Name
MYH14
Full Name
Myosin-14
Weight
227871 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Myosin-14;Myosin heavy chain 14;Myosin heavy chain, non-muscle IIc;Non-muscle myosin heavy chain IIc;NMHC II-C;MYH14;KIAA2034;FP17425; Myh14|2400004E04Rik, II-C, NHMCII, NMHC II-C|myosin, heavy polypeptide 14|myosin-14|myosin heavy chain, non-muscle IIc|myosin, heavy chain 14|non-muscle myosin heavy chain IIc|nonmuscle myosin heavy chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYH14, check out the MYH14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYH14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYH14/Myosin Heavy Chain Antibody (A04528)
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No publications found for A04528
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-MYH14/Myosin Heavy Chain Antibody
Question
My question regarding product A04528, anti-MYH14/Myosin Heavy Chain antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04528 anti-MYH14/Myosin Heavy Chain antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-MYH14/Myosin Heavy Chain antibody A04528. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
Will anti-MYH14/Myosin Heavy Chain antibody A04528 work on feline WB with lymph node?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Our lab technicians have not validated anti-MYH14/Myosin Heavy Chain antibody A04528 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MYH14/Myosin Heavy Chain antibody A04528 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lymph node in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-MYH14/Myosin Heavy Chain antibody for WB for human lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
It shows on the product datasheet, A04528 anti-MYH14/Myosin Heavy Chain antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-05
Question
Will anti-MYH14/Myosin Heavy Chain antibody A04528 work for WB with lymph node?
C. Collins
Verified customer
Asked: 2019-02-27
Answer
According to the expression profile of lymph node, MYH14 is highly expressed in lymph node. So, it is likely that anti-MYH14/Myosin Heavy Chain antibody A04528 will work for WB with lymph node.
Boster Scientific Support
Answered: 2019-02-27
Question
Is there a BSA free version of anti-MYH14/Myosin Heavy Chain antibody A04528 available?
L. Evans
Verified customer
Asked: 2019-02-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MYH14/Myosin Heavy Chain antibody A04528 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-27
Question
I see that the anti-MYH14/Myosin Heavy Chain antibody A04528 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-07
Question
My question regards to test anti-MYH14/Myosin Heavy Chain antibody A04528 on human lymph node for research purposes, then I may be interested in using anti-MYH14/Myosin Heavy Chain antibody A04528 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
The products we sell, including anti-MYH14/Myosin Heavy Chain antibody A04528, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-23
Question
See attached the WB image, lot number and protocol we used for lymph node using anti-MYH14/Myosin Heavy Chain antibody A04528. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-17
Question
Does A04528 anti-MYH14/Myosin Heavy Chain antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Jones
Verified customer
Asked: 2017-07-10
Answer
As indicated on the product datasheet, A04528 anti-MYH14/Myosin Heavy Chain antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-10
Question
Is this A04528 anti-MYH14/Myosin Heavy Chain antibody reactive to the isotypes of MYH14?
K. Dhar
Verified customer
Asked: 2015-07-29
Answer
The immunogen of A04528 anti-MYH14/Myosin Heavy Chain antibody is Synthesized peptide derived from internal of human MYH14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-07-29
Question
Is a blocking peptide available for product anti-MYH14/Myosin Heavy Chain antibody (A04528)?
J. Jha
Verified customer
Asked: 2014-12-19
Answer
We do provide the blocking peptide for product anti-MYH14/Myosin Heavy Chain antibody (A04528). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-19
