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Product Info Summary
| SKU: | M00211-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Cow, Human, Mouse, Pig, Rat, Horse |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Myelin Basic Protein MBP Monoclonal Antibody
SKU/Catalog Number
M00211-1
Size
100ul
Description
Boster Bio Anti-Myelin Basic Protein MBP Monoclonal Antibody catalog # M00211-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Cow, Human, Mouse, Pig, Rat, Horse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myelin Basic Protein MBP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00211-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Purified myelin basic protein isolated from bovine brain
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00211-1 is reactive to Mbp in Cow, Human, Mouse, Pig, Rat, Horse
Reconstitution
Calculated molecular weight
27168 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00211-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10,000 -1:20,000. IF/ICC 1:2,000-5,000. IHC: 1:10,000.
Validation Images & Assay Conditions
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Western blot analysis of different tissue lysates using mouse mAb to MBP, M00211-1, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.
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Immunofluorescent analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), M00211-1, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H, RPCA-NF-H, dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.
Protein Target Info & Infographic
Gene/Protein Information For Mbp (Source: Uniprot.org, NCBI)
Gene Name
Mbp
Full Name
Myelin basic protein
Weight
27168 MW
Superfamily
myelin basic protein family
Alternative Names
Myelin basic protein;MBP;Myelin A1 protein;Mbp;Shi; MBP myelin basic protein myelin basic protein|Golli-MBP|proline-rich proteoglycan 2|myelin A1 protein|myelin membrane encephalitogenic protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mbp, check out the Mbp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mbp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myelin Basic Protein MBP Monoclonal Antibody (M00211-1)
Hello CJ!
M00211-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng FW,Jing XN,Song GH,Jie LL,Shen FF.Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury.J Anat.2020 Nov;237(5):933-940.doi: 10.1111/joa.13247.Epub 2020 Jun 9.PMID:32515838;PMCID:
Species: Mouse
M00211-1 usage in article: APP:IF, SAMPLE:SCIATIC NERVE TISSUE, DILUTION:1:100
Nexilin Regulates Oligodendrocyte Progenitor Cell Migration and Remyelination and is Negatively Regulated by PAR1/RAP1 Signaling Following Subarachnoid %u2026
Jin Sj, Liu Y, Deng Sh, Liao Lh, Lin Tl, Ning Q, Luo Xp. Int J Mol Med. 2015 May;35(5):1199-212. Doi: 10.3892/Ijmm.2015.2136. Epub 2015 Mar 13. Neuroprotective Effects Of Activated Protein C On Intrauterine Inflammation-Induced Neonatal White Matt...
Han Sm, Wan H, Kudo G, Foltz Wd, Vines Dc, Green De, Zoerle T, Tariq A, Brathwaite S, D'Abbondanza J, Ai J, Macdonald Rl. J Cereb Blood Flow Metab. 2014 Jan;34(1):108-17. Doi: 10.1038/Jcbfm.2013.170. Epub 2013 Sep 25. Molecular Alterations In The ...
Yang P, Gao Z, Zhang H, Fang Z, Wu C, Xu H, Huang Qj. Neuropsychiatr Dis Treat. 2015 Mar 6;11:597-607. Doi: 10.2147/Ndt.S78131. Ecollection 2015. Changes In Proinflammatory Cytokines And White Matter In Chronically Stressed Rats.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Myelin Basic Protein MBP Monoclonal Antibody
Question
I was wanting to use your anti-Myelin Basic Protein MBP Monoclonal antibody for IF for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
As indicated on the product datasheet, M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody has been validated for IF, WB on bovine, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
Do you have a BSA free version of anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 available?
T. Wu
Verified customer
Asked: 2019-10-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-29
Question
We have seen staining in pig c1 segment of cervical spinal cord. Are there any suggestions? Is anti-Myelin Basic Protein MBP Monoclonal antibody supposed to stain c1 segment of cervical spinal cord positively?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
From what I have seen in literature c1 segment of cervical spinal cord does express MBP. From what I have seen in Uniprot.org, MBP is expressed in c1 segment of cervical spinal cord, embryonic spinal cord, brain, amygdala, hippocampus subthalamic nucleus, brain, lung skin, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-10-04
Question
Is this M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody reactive to the isotypes of MBP?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The immunogen of M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody is Purified myelin basic protein isolated from bovine brain. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-30
Question
Is a blocking peptide available for product anti-Myelin Basic Protein MBP Monoclonal antibody (M00211-1)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do provide the blocking peptide for product anti-Myelin Basic Protein MBP Monoclonal antibody (M00211-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The anti-Myelin Basic Protein MBP Monoclonal antibody (M00211-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
Would anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
According to the expression profile of leukemic t-cell, MBP is highly expressed in leukemic t-cell. So, it is likely that anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-05-22
Question
My lab would like to test anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
The products we sell, including anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-18
Question
We bought anti-Myelin Basic Protein MBP Monoclonal antibody for WB on hippocampus subthalamic nucleus a few years ago. I am using human, and We are going to use the antibody for IF next. I am interested in examining hippocampus subthalamic nucleus as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
I took a look at the website and datasheets of our anti-Myelin Basic Protein MBP Monoclonal antibody and I see that M00211-1 has been tested on human in both WB and IF. Thus M00211-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-07
Question
Would M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Williams
Verified customer
Asked: 2017-10-16
Answer
It shows on the product datasheet, M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-16
Question
I see that the anti-Myelin Basic Protein MBP Monoclonal antibody M00211-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-28
Question
My question regarding product M00211-1, anti-Myelin Basic Protein MBP Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Wu
Verified customer
Asked: 2017-07-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-14
Question
I would like using your anti-Myelin Basic Protein MBP Monoclonal antibody for response to toxic substance studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
J. Collins
Verified customer
Asked: 2016-12-06
Answer
Thanks for your inquiry. This M00211-1 anti-Myelin Basic Protein MBP Monoclonal antibody is validated on mouse brain. It is guaranteed to work for IF, WB in bovine, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-12-06
Question
My colleagues were satisfied with the WB result of your anti-Myelin Basic Protein MBP Monoclonal antibody. However we have seen positive staining in amygdala myelin membrane using this antibody. Is that expected? Could you tell me where is MBP supposed to be expressed?
J. Miller
Verified customer
Asked: 2014-05-07
Answer
From what I have seen in literature, amygdala does express MBP. Generally MBP expresses in myelin membrane. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2014-05-07
