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Product Info Summary
| SKU: | M00025-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306
SKU/Catalog Number
M00025-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306 (Catalog # M00025-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00025-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM306
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human MyD88
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human myeloid differentiation primary response protein MyD88
Reactive Species
M00025-1 is reactive to MYD88 in Human
Reconstitution
Calculated molecular weight
33233 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00025-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:250 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of K562 cells lysate using anti-MyD88 rabbit monoclonal antibody (Clone RM306) at a 1:100 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-MyD88 rabbit monoclonal antibody (Clone RM306) at a 1:250 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MYD88 (Source: Uniprot.org, NCBI)
Gene Name
MYD88
Full Name
Myeloid differentiation primary response protein MyD88
Weight
33233 MW
Alternative Names
Myeloid differentiation primary response protein MyD88;MYD88; MYD88 IMD68D, MYD88 MYD88 innate immune signal transduction adaptor myeloid differentiation primary response protein MyD88|mutant myeloid differentiation primary response 88|myeloid differentiation primary response 88|myeloid differentiation primary response gene (88)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYD88, check out the MYD88 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYD88: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306 (M00025-1)
Hello CJ!
M00025-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306
Question
Is a blocking peptide available for product anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 (M00025-1)?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
We do provide the blocking peptide for product anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 (M00025-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-07
Question
See attached the WB image, lot number and protocol we used for pancreas using anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use your anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 for WB for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
It shows on the product datasheet, M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-09
Question
My question regards using your anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 for pp2a and ier3 regulate pi3k/akt signaling studies. Has this antibody been tested with western blotting on human kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Thanks for your inquiry. This M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 is tested on k562 cells, human kidney tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-18
Question
We are currently using anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
L. Wu
Verified customer
Asked: 2020-02-11
Answer
The anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 (M00025-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-11
Question
Is there a BSA free version of anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 available?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-20
Question
My team were content with the WB result of your anti-MYD88 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
From what I have seen in literature, erythroleukemia does express MYD88. Generally MYD88 expresses in cytoplasm. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-20
Question
I would like to test anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 on human pancreas for research purposes, then I may be interested in using anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The products we sell, including anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-22
Question
We have been able to see staining in human erythroleukemia. What should we do? Is anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Based on literature erythroleukemia does express MYD88. Based on Uniprot.org, MYD88 is expressed in blood, dendritic cell, epidermal carcinoma, umbilical cord blood, pancreas, erythroleukemia, among other tissues. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-04
Question
I see that the anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-20
Question
Is this M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 reactive to the isotypes of MYD88?
J. Bhatt
Verified customer
Asked: 2019-02-01
Answer
The immunogen of M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 is A peptide corresponding to the N-terminus of human MyD88. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-01
Question
Does M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
You can see on the product datasheet, M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-17
Question
Does anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 work for WB with pancreas?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
According to the expression profile of pancreas, MYD88 is highly expressed in pancreas. So, it is likely that anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306 M00025-1 will work for WB with pancreas.
Boster Scientific Support
Answered: 2017-11-07
Question
We ordered your anti-MYD88 Rabbit Monoclonal antibody, Clone
H. Zhang
Verified customer
Asked: 2016-09-22
Answer
I have checked the website and datasheets of our anti-MYD88 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2016-09-22
Question
I have a question about product M00025-1, anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jha
Verified customer
Asked: 2016-07-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00025-1 anti-MYD88 Rabbit Monoclonal antibody, Clone#RM306, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-05
