Product Info Summary
SKU: | M34008 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MUTA Rabbit Monoclonal Antibody
SKU/Catalog Number
M34008
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-MUTA Rabbit Monoclonal Antibody catalog # M34008. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUTA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M34008)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27M55
Isotype
IgG
Immunogen
A synthesized peptide derived from human MUTA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M34008 is reactive to MMUT in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
83 kDa
Calculated molecular weight
26053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M34008 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human K562 whole cell, human 293T whole cell, human PC-3 whole cell, rat PC-12 whole cell, rat kidney tissue, mouse NIH/3T3 whole cell, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MMUT using anti-MMUT antibody (M34008).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMUT antigen affinity purified monoclonal antibody (Catalog # M34008) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMUT at approximately 83 kDa. The expected band size for MMUT is at 83 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MMUT (Source: Uniprot.org, NCBI)
Gene Name
MMUT
Full Name
Methylmalonyl-CoA mutase, mitochondrial
Weight
26053 MW
Superfamily
methylmalonyl-CoA mutase family
Alternative Names
Red fluorescent protein eqFP611;GFP-like chromoprotein; MMUT MCM, MUT methylmalonyl-CoA mutase methylmalonyl-CoA mutase, mitochondrial|methylmalonyl Coenzyme A mutase|methylmalonyl-CoA isomerase|methylmalonyl-CoA mutase c.*192delA|methylmalonyl-CoA mutase c.*51C>G|methylmalonyl-CoA mutase variant c.1495G>A|methylmalonyl-CoA mutase variant c.2011A>G|methylmalonyl-CoA mutase variant c.2150G>T|methylmalonyl-CoA mutase variant c.322C>T|methylmalonyl-CoA mutase variant c.613_615delGAA|methylmalonyl-CoA mutase variant c.636G>A|methylmalonyl-CoA mutase variant c.643G>A|mutant methylmalonyl CoA mutase|truncated methylmalonyl CoA mutase|truncated methylmalonyl-CoA mutase variant c.1420C>T|truncated methylmalonyl-CoA mutase variant c.2179C>T|truncated methylmalonyl-CoA mutase variant c.91C>T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMUT, check out the MMUT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMUT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUTA Rabbit Monoclonal Antibody (M34008)
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