Anti-Phospho-Munc-18 (Ser513) STXBP2 Antibody

Product Name

Anti-Phospho-Munc-18 (Ser513) STXBP2 Antibody

View all Syntaxin-BP2 Antibodies

SKU/Catalog Number

P04909

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Munc-18 (Ser513) STXBP2 Antibody (Catalog # P04909). Tested in WB, ICC applications. This antibody reacts with Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Munc-18 (Ser513) STXBP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04909)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P04909 is reactive to STXBP2 in Rat

Applications

P04909 is guaranteed for ICC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

66453 MW

Background of Syntaxin-BP2

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser-515 in C. Elegans (Sassa et al., 1996). Ser-513 is the mammalian homologue of Ser-515 in C. Elegans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
ICC: 1:400

Validation Images & Assay Conditions

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Immunostaining of 14 DIV rat cortical neurons showing specific labeling of Munc-18 phosphorylated at Ser⁵¹³ (red) and PSD95 (green).

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Western blot of rat cortical lysate showing specific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser⁵¹³ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For STXBP2 (Source: Uniprot.org, NCBI)

Alternative Names

FHL5; Hunc18b; MUNC18-2; Protein unc-18 homolog 2; Protein unc-18 homolog B; STXBP2; syntaxin binding protein 2; syntaxin-binding protein 2; SyntaxinBP2; Syntaxin-BP2; Unc18-2; UNC18B; unc-18B; UNC18Bpp10122 STXBP2 FHL5, Hunc18b, MUNC18-2, UNC18-2, UNC18B, pp10122 syntaxin binding protein 2 syntaxin-binding protein 2|protein unc-18 homolog B|unc-18B

For more info on STXBP2, check out the STXBP2 Infographic

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In this infographic, you will see the following information for STXBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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