Anti-MUC20 Antibody Picoband®

MUC20 antibody

Boster Bio Anti-MUC20 Antibody Picoband® catalog # A07372. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A07372
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-MUC20 Antibody Picoband®

View all MUC20 Antibodies

SKU/Catalog Number

A07372

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MUC20 Antibody Picoband® catalog # A07372. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MUC20 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07372)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MUC20 recombinant protein (Position: S28-G709).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07372 is reactive to MUC20 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

75 kDa

Calculated molecular weight

108320 MW

Background of MUC20

Mucin-20 is a protein that in humans is encoded by the MUC20 gene. This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07372 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human HEK293 whole cell, human A549 whole cell, uman HL-60 whole cell, uman K562 whole cell, rat kidney tissue, mouse kidney tissue,
IHC: human placenta tissue, mouse kidney tissue

Validation Images & Assay Conditions

Gene/Protein Information For MUC20 (Source: Uniprot.org, NCBI)

Gene Name

MUC20

Full Name

Mucin-20

Weight

108320 MW

Alternative Names

Mucin-20; MUC-20; MUC20; KIAA1359; UNQ2782/PRO7170 MUC20 MUC-20 mucin 20, cell surface associated mucin-20|transmembrane mucin MUC20S

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MUC20, check out the MUC20 Infographic

MUC20 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MUC20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MUC20 Antibody Picoband®

Question

Can you help my question with product A07372, anti-MUC20 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07372 anti-MUC20 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-10

Question

We are currently using anti-MUC20 antibody A07372 for rat tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

The anti-MUC20 antibody (A07372) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-08

Question

Would A07372 anti-MUC20 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

You can see on the product datasheet, A07372 anti-MUC20 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-25

Question

Is this A07372 anti-MUC20 antibody reactive to the isotypes of MUC20?

F. Huang

Verified customer

Asked: 2018-09-24

Answer

The immunogen of A07372 anti-MUC20 antibody is E.coli-derived human MUC20 recombinant protein (Position: S28-G709). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-24

Question

Would anti-MUC20 antibody A07372 work on goat WB with right uterine tube?

C. Banerjee

Verified customer

Asked: 2013-11-04

Answer

Our lab technicians have not validated anti-MUC20 antibody A07372 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MUC20 antibody A07372 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat right uterine tube in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-11-04

Order DetailsPrice
A07372

100μg

$370
A07372-10ug

10μg sample (liquid)

$99
A07372-Biotin

100 μg Biotin conjugated

$570
A07372-Cy3

100 μg Cy3 conjugated

$570
A07372-Dylight488

100 μg Dylight488 conjugated

$570
A07372-Dylight550

100 μg Dylight550 conjugated

$570
A07372-Dylight594

100 μg Dylight594 conjugated

$570
A07372-FITC

100 μg FITC conjugated

$570
A07372-HRP

100 μg HRP conjugated

$570
A07372-APC

100 μg APC conjugated

$670
A07372-PE

100 μg PE conjugated

$670
A07372-iFluor647

100 μg iFluor647 conjugated

$670
A07372-carrier-free

Carrier Free

$370

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A07372
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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