Product Info Summary
SKU: | PA1317 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MTCO1/MT-CO1 Antibody Picoband®
SKU/Catalog Number
PA1317
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MTCO1/MT-CO1 Antibody catalog # PA1317. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MTCO1/MT-CO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1317)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1317 is reactive to MT-CO1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
57041 MW
Background of MT-CO1
Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1317 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue, human COLO320 whole cell, human SMMC whole cell, human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MTCO1 using anti-MTCO1 antibody (PA1317).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human SMMC whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37 kDa. The expected band size for MTCO1 is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MT-CO1 (Source: Uniprot.org, NCBI)
Gene Name
MT-CO1
Full Name
Cytochrome c oxidase subunit 1
Weight
57041 MW
Superfamily
heme-copper respiratory oxidase family
Alternative Names
Cytochrome c oxidase subunit 1;1.9.3.1;Cytochrome c oxidase polypeptide I;MT-CO1;COI, COXI, MTCO1;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MT-CO1, check out the MT-CO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MT-CO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MTCO1/MT-CO1 Antibody Picoband® (PA1317)
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No publications found for PA1317
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-MTCO1/MT-CO1 Antibody Picoband®
Question
Does PA1317 anti-MTCO1/MT-CO1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can see on the product datasheet, PA1317 anti-MTCO1/MT-CO1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-19
Question
I have a question about product PA1317, anti-MTCO1/MT-CO1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1317 anti-MTCO1/MT-CO1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-26
Question
Does anti-MTCO1/MT-CO1 antibody PA1317 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
According to the expression profile of placenta, MT-CO1 is highly expressed in placenta. So, it is likely that anti-MTCO1/MT-CO1 antibody PA1317 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-06-12
Question
I was wanting to use to test anti-MTCO1/MT-CO1 antibody PA1317 on human placenta for research purposes, then I may be interested in using anti-MTCO1/MT-CO1 antibody PA1317 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
The products we sell, including anti-MTCO1/MT-CO1 antibody PA1317, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-21
Question
Is a blocking peptide available for product anti-MTCO1/MT-CO1 antibody (PA1317)?
K. Wu
Verified customer
Asked: 2016-12-23
Answer
We do provide the blocking peptide for product anti-MTCO1/MT-CO1 antibody (PA1317). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-23
Question
Do you have a BSA free version of anti-MTCO1/MT-CO1 antibody PA1317 available?
W. Collins
Verified customer
Asked: 2014-02-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MTCO1/MT-CO1 antibody PA1317 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-02-03