Anti-MTCO1/MT-CO1 Antibody Picoband®

MT-CO1 antibody

Boster Bio Anti-MTCO1/MT-CO1 Antibody catalog # PA1317. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA1317
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-MTCO1/MT-CO1 Antibody Picoband®

View all MT-CO1 Antibodies

SKU/Catalog Number

PA1317

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MTCO1/MT-CO1 Antibody catalog # PA1317. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MTCO1/MT-CO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1317)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1317 is reactive to MT-CO1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

57041 MW

Background of MT-CO1

Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1317 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: human placenta tissue, human COLO320 whole cell, human SMMC whole cell, human Hela whole cell

Validation Images & Assay Conditions

Gene/Protein Information For MT-CO1 (Source: Uniprot.org, NCBI)

Gene Name

MT-CO1

Full Name

Cytochrome c oxidase subunit 1

Weight

57041 MW

Superfamily

heme-copper respiratory oxidase family

Alternative Names

Cytochrome c oxidase subunit 1;1.9.3.1;Cytochrome c oxidase polypeptide I;MT-CO1;COI, COXI, MTCO1;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MT-CO1, check out the MT-CO1 Infographic

MT-CO1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MT-CO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-MTCO1/MT-CO1 Antibody Picoband®

Question

Does PA1317 anti-MTCO1/MT-CO1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

You can see on the product datasheet, PA1317 anti-MTCO1/MT-CO1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-19

Question

I have a question about product PA1317, anti-MTCO1/MT-CO1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1317 anti-MTCO1/MT-CO1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-26

Question

Does anti-MTCO1/MT-CO1 antibody PA1317 work for WB with placenta?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

According to the expression profile of placenta, MT-CO1 is highly expressed in placenta. So, it is likely that anti-MTCO1/MT-CO1 antibody PA1317 will work for WB with placenta.

Boster Scientific Support

Answered: 2019-06-12

Question

I was wanting to use to test anti-MTCO1/MT-CO1 antibody PA1317 on human placenta for research purposes, then I may be interested in using anti-MTCO1/MT-CO1 antibody PA1317 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

The products we sell, including anti-MTCO1/MT-CO1 antibody PA1317, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-21

Question

Is a blocking peptide available for product anti-MTCO1/MT-CO1 antibody (PA1317)?

K. Wu

Verified customer

Asked: 2016-12-23

Answer

We do provide the blocking peptide for product anti-MTCO1/MT-CO1 antibody (PA1317). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-12-23

Question

Do you have a BSA free version of anti-MTCO1/MT-CO1 antibody PA1317 available?

W. Collins

Verified customer

Asked: 2014-02-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MTCO1/MT-CO1 antibody PA1317 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-02-03

Order DetailsPrice
PA1317

100μg

$370
PA1317-10ug

10μg sample (liquid)

$99
PA1317-Biotin

100 μg Biotin conjugated

$570
PA1317-Cy3

100 μg Cy3 conjugated

$570
PA1317-Dylight488

100 μg Dylight488 conjugated

$570
PA1317-Dylight550

100 μg Dylight550 conjugated

$570
PA1317-Dylight594

100 μg Dylight594 conjugated

$570
PA1317-FITC

100 μg FITC conjugated

$570
PA1317-HRP

100 μg HRP conjugated

$570
PA1317-APC

100 μg APC conjugated

$670
PA1317-PE

100 μg PE conjugated

$670
PA1317-iFluor647

100 μg iFluor647 conjugated

$670
PA1317-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1317
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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