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Product Info Summary
| SKU: | A09552-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MST4/STK26 Antibody Picoband®
SKU/Catalog Number
A09552-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MST4/STK26 Antibody Picoband® catalog # A09552-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MST4/STK26 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09552-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MST4/STK26 recombinant protein (Position: K233-P416).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09552-2 is reactive to STK26 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
46.529kDa
Background of STK26
The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09552-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, rat PC-12 whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MST4/STK26 using anti-MST4/STK26 antibody (A09552-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST4/STK26 antigen affinity purified polyclonal antibody (Catalog # A09552-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST4/STK26 at approximately 52 kDa. The expected band size for MST4/STK26 is at 47 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-MST4/STK26 antibody (A09552-2).
Overlay histogram showing MCF-7 cells stained with A09552-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MST4/STK26 Antibody (A09552-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For STK26 (Source: Uniprot.org, NCBI)
Gene Name
STK26
Full Name
Serine/threonine-protein kinase 26
Weight
46.529kDa
Superfamily
protein kinase superfamily
Alternative Names
Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP; STK26 MASK, MST4 serine/threonine kinase 26 serine/threonine-protein kinase 26|Mst3 and SOK1-related kinase|STE20-like kinase 4|STE20-like kinase MST4|mammalian Ste20-like protein kinase 4|mammalian sterile 20-like 4|serine/threonine protein kinase 26|serine/threonine protein kinase MST4|serine/threonine-protein kinase MASK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STK26, check out the STK26 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STK26: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MST4/STK26 Antibody Picoband® (A09552-2)
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