Anti-MSI2 Antibody

Product Name

Anti-MSI2 Antibody

View all Musashi-2 Antibodies

SKU/Catalog Number

A02898

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MSI2 Antibody (Catalog # A02898). Tested in ELISA, WB, ICC, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MSI2 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MSI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02898)

Host

Rabbit

Contents

MSI2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MSI2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2. The immunogen is located within amino acids 30 - 80 of MSI2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.

Reactive Species

A02898 is reactive to Msi2 in Human, Mouse, Rat

Applications

A02898 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

35197 MW

Background of Musashi-2

Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MSI2 antibody can be used for detection of MSI2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 μg/mL.

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Immunocytochemistry of MSI2 in EL4 cells with MSI2 antibody at 5 μg/mL.

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Immunofluorescence of MSI2 in EL4 cells with MSI2 antibody at 20 μg/mL.

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Immunohistochemistry of MSI2 in mouse brain tissue with MSI2 Antibody at 5 μg/mL.

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Immunofluorescence of MSI2 in mouse brain tissue with MSI2 Antibody at 20 μg/mL.

Green: MSI2 antibody (A02898)
Red: Phylloidin staining
Blue: DAPI staining

Gene/Protein Information For Msi2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ36569; MGC3245; MSI2; MSI2H; musashi homolog 2 (Drosophila); Musashi2; Musashi-2; RNA-binding protein Musashi homolog 2 Msi2|1700105C15Rik, AI451722, AI563628, AW489193h, Musa, msi, Msi2|musashi RNA-binding protein 2|RNA-binding protein Musashi homolog 2|Musashi2|musashi-2

For more info on Msi2, check out the Msi2 Infographic

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In this infographic, you will see the following information for Msi2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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