Anti-MRPS27 Antibody

Product Name

Anti-MRPS27 Antibody

View all MRPS27 Antibodies

SKU/Catalog Number

A12929

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPS27 Antibody (Catalog # A12929). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPS27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12929)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPS27.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12929 is reactive to MRPS27 in Human, Mouse

Reconstitution

Calculated molecular weight

47611 MW

Background of MRPS27

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms.

Nagase T., DNA Res. 3:321-329(1996).
Koc E.C., Protein Sci. 10:471-481(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12929 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For MRPS27 (Source: Uniprot.org, NCBI)

Alternative Names

28S ribosomal protein S27, mitochondrial;MRP-S27;S27mt;MRPS27;KIAA0264; MRPS27 MRP-S27, S27mt mitochondrial ribosomal protein S27 28S ribosomal protein S27, mitochondrial|mitochondrial 28S ribosomal protein S27|mitochondrial small ribosomal subunit protein mS27

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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