Anti-MRPS21 Antibody

Product Name

Anti-MRPS21 Antibody

View all MRPS21 Antibodies

SKU/Catalog Number

A14514

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPS21 Antibody (Catalog # A14514). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPS21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14514)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPS21.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14514 is reactive to MRPS21 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

10742 MW

Background of MRPS21

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein.

Suzuki T., J. Biol. Chem. 276:33181-33195(2001).
Huang C., Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14514 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MRPS21 (Source: Uniprot.org, NCBI)

Alternative Names

28S ribosomal protein S21, mitochondrial;MRP-S21;S21mt;MRPS21;RPMS21;MDS016; MRPS21 MDS016, MRP-S21, RPMS21 mitochondrial ribosomal protein S21 28S ribosomal protein S21, mitochondrial|S21mt|mitochondrial 28S ribosomal protein S21|mitochondrial small ribosomal subunit protein bS21m

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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