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Product Info Summary
| SKU: | A14080-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MRP-S25 Antibody
SKU/Catalog Number
A14080-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-S25 Antibody catalog # A14080-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14080-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human MRP-S25. at AA rangle: 110-190
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14080-1 is reactive to MRPS25 in Human, Mouse, Rat
Applications
A14080-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
20116 MW
Background of MRPS25
RNA binding motif protein 47 is a protein in humans that is encoded by the RBM47 gene in chromosome 4. RBM47 may be involved in RNA binding and nucleotide binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using MRP-S25 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For MRPS25 (Source: Uniprot.org, NCBI)
Gene Name
MRPS25
Full Name
28S ribosomal protein S25, mitochondrial
Weight
20116 MW
Superfamily
mitochondrion-specific ribosomal protein mS25 family
Alternative Names
FLJ00023; mitochondrial 28S ribosomal protein S25; mitochondrial ribosomal protein S25; MRP-S2528S ribosomal protein S25, mitochondrial; RPMS25DKFZp313H0817; S25mt MRPS25 COXPD50, MRP-S25, RPMS25 mitochondrial ribosomal protein S25 28S ribosomal protein S25, mitochondrial|S25mt|mitochondrial 28S ribosomal protein S25|mitochondrial small ribosomal subunit protein mS25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPS25, check out the MRPS25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPS25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-S25 Antibody (A14080-1)
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No publications found for A14080-1
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Customer Q&As
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5 Customer Q&As for Anti-MRP-S25 Antibody
Question
Does A14080-1 anti-MRP-S25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, A14080-1 anti-MRP-S25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-03
Question
Is this A14080-1 anti-MRP-S25 antibody reactive to the isotypes of MRPS25?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The immunogen of A14080-1 anti-MRP-S25 antibody is Synthesized peptide derived from the C-terminal region of human MRP-S25. at AA rangle: 110-190. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-MRP-S25 antibody A14080-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
The anti-MRP-S25 antibody (A14080-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-05
Question
I have a question about product A14080-1, anti-MRP-S25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14080-1 anti-MRP-S25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-25
Question
I am looking for to test anti-MRP-S25 antibody A14080-1 on rat left lobe of thyroid gland for research purposes, then I may be interested in using anti-MRP-S25 antibody A14080-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Anderson
Verified customer
Asked: 2017-11-20
Answer
The products we sell, including anti-MRP-S25 antibody A14080-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-20
