Anti-MRP-S25 Antibody

MRPS25 antibody

Boster Bio Anti-MRP-S25 Antibody catalog # A14080-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A14080-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-MRP-S25 Antibody

View all MRPS25 Antibodies

SKU/Catalog Number

A14080-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MRP-S25 Antibody catalog # A14080-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRP-S25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14080-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14080-1 is reactive to MRPS25 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

20116 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14080-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MRPS25 (Source: Uniprot.org, NCBI)

Gene Name

MRPS25

Full Name

28S ribosomal protein S25, mitochondrial

Weight

20116 MW

Superfamily

mitochondrion-specific ribosomal protein mS25 family

Alternative Names

28S ribosomal protein S25, mitochondrial;MRP-S25;S25mt;MRPS25;RPMS25; MRPS25 COXPD50, MRP-S25, RPMS25 mitochondrial ribosomal protein S25 28S ribosomal protein S25, mitochondrial|S25mt|mitochondrial 28S ribosomal protein S25|mitochondrial small ribosomal subunit protein mS25

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MRPS25, check out the MRPS25 Infographic

MRPS25 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRPS25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MRP-S25 Antibody

Question

Does A14080-1 anti-MRP-S25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

You can see on the product datasheet, A14080-1 anti-MRP-S25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-03

Question

Is this A14080-1 anti-MRP-S25 antibody reactive to the isotypes of MRPS25?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

The immunogen of A14080-1 anti-MRP-S25 antibody is Synthesized peptide derived from the C-terminal region of human MRP-S25. at AA rangle: 110-190. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-09

Question

We are currently using anti-MRP-S25 antibody A14080-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-05

Answer

The anti-MRP-S25 antibody (A14080-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-05

Question

I have a question about product A14080-1, anti-MRP-S25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-25

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14080-1 anti-MRP-S25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-25

Question

I am looking for to test anti-MRP-S25 antibody A14080-1 on rat left lobe of thyroid gland for research purposes, then I may be interested in using anti-MRP-S25 antibody A14080-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Anderson

Verified customer

Asked: 2017-11-20

Answer

The products we sell, including anti-MRP-S25 antibody A14080-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-20

Order DetailsPrice
A14080-1

100uL

$399

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A14080-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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