Anti-(Mouse) Med15 Antibody (C-term)

Product Name

Anti-(Mouse) Med15 Antibody (C-term)

View all MED15 Antibodies

SKU/Catalog Number

M03568-1

Size

80 µl

Description

Boster Bio Anti-(Mouse) Med15 Antibody (C-term) (Catalog # M03568-1). Tested in WB, IHC-P application(s). This antibody reacts with Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-(Mouse) Med15 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03568-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03568-1 is reactive to MED15 in Mouse, Rat

Applications

M03568-1 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

86607 Da

Background of MED15

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P: 1:25

Validation Images & Assay Conditions

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Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 87 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilution
Lane 1: mouse brain lysates
Lane 2: mouse spleen lysates
Lane 3: mouse thymus lysates
Lane 4: rat lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 87 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

M03568-1 staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For MED15 (Source: Uniprot.org, NCBI)

Alternative Names

ARC105Arc105; CAG7A; CTG repeat protein 7a; CTG7A; FLJ42282; FLJ42935; mediator complex subunit 15PC2 glutamine/Q-rich-associated protein; mediator of RNA polymerase II transcription subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein; PC2-glutamine-rich-associated protein; PCQAPDKFZp686A2214; Positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1DKFZp762B1216; TIG-1TPA-inducible gene 1 protein; TNRC7; TPA inducible gene-1; TPA inducible protein; trinucleotide repeat containing 7,105-kD; MED15 ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7 mediator complex subunit 15 mediator of RNA polymerase II transcription subunit 15|CTG repeat protein 7a|PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein|PC2 glutamine/Q-rich-associated protein|PC2-glutamine-rich-associated protein|TPA inducible gene-1|TPA inducible protein|TPA-inducible gene 1 protein|activator-recruited cofactor 105 kDa component|activator-recruited cofactor, 105-kD|positive cofactor 2, glutamine/Q-rich-associated protein|trinucleotide repeat containing 7|trinucleotide repeat-containing gene 7 protein

For more info on MED15, check out the MED15 Infographic

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In this infographic, you will see the following information for MED15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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