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Product Info Summary
| SKU: | M03568-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-(Mouse) Med15 Antibody (C-term)
SKU/Catalog Number
M03568-1
Size
80 µl
Description
Boster Bio Anti-(Mouse) Med15 Antibody (C-term) (Catalog # M03568-1). Tested in WB, IHC-P application(s). This antibody reacts with Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Med15 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03568-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03568-1 is reactive to Med15 in Mouse, Rat
Reconstitution
Calculated molecular weight
86607 Da
Background of MED15
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03568-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 87 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilution
Lane 1: mouse brain lysates
Lane 2: mouse spleen lysates
Lane 3: mouse thymus lysates
Lane 4: rat lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 87 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
M03568-1 staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For Med15 (Source: Uniprot.org, NCBI)
Gene Name
Med15
Full Name
Mediator of RNA polymerase II transcription subunit 15
Weight
86607 Da
Superfamily
Mediator complex subunit 15 family
Alternative Names
Mediator of RNA polymerase II transcription subunit 15, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, mPcqap, Med15, Pcqap MED15 ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7 mediator complex subunit 15 mediator of RNA polymerase II transcription subunit 15|CTG repeat protein 7a|PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein|PC2 glutamine/Q-rich-associated protein|PC2-glutamine-rich-associated protein|TPA inducible gene-1|TPA inducible protein|TPA-inducible gene 1 protein|activator-recruited cofactor 105 kDa component|activator-recruited cofactor, 105-kD|positive cofactor 2, glutamine/Q-rich-associated protein|trinucleotide repeat containing 7|trinucleotide repeat-containing gene 7 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Med15, check out the Med15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Med15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-(Mouse) Med15 Antibody (C-term) (M03568-1)
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