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Product Info Summary
| SKU: | A06075-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Morc3 Antibody
SKU/Catalog Number
A06075-1
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Morc3 Antibody (Catalog # A06075-1). Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Morc3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06075-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Morc3 whole rabbit serum was prepared by repeated immunizations with a mouse Morc3 recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06075-1 is reactive to MORC3 in Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
107569 MW
Background of MORC3
The Morc (microrchidia) family of proteins are ATPases of the GHKL family. They have been implicated in transcriptional repression of genes and transposons, and higher order organization of DNA within the nucleus. Morc antibodies are ideal for researchers interested in Epigenetics, Cancer, and Cell cycle research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06075-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000-1:50,000
WB: 1:1000-1:5000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Morc3 using anti-Morc3 antibody (A06075-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Morc3 antigen affinity purified polyclonal antibody (Catalog # A06075-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Morc3.
Protein Target Info & Infographic
Gene/Protein Information For MORC3 (Source: Uniprot.org, NCBI)
Gene Name
MORC3
Full Name
MORC family CW-type zinc finger protein 3
Weight
107569 MW
Alternative Names
Morc3, MORC family CW-type zinc finger protein 3, Protein microrchidia, Morc MORC3 NXP2, ZCW5, ZCWCC3 MORC family CW-type zinc finger 3 MORC family CW-type zinc finger protein 3|nuclear matrix protein 2|nuclear matrix protein NXP2|zinc finger CW-type coiled-coil domain protein 3|zinc finger, CW type with coiled-coil domain 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MORC3, check out the MORC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MORC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Morc3 Antibody (A06075-1)
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Customer Q&As
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5 Customer Q&As for Anti-Morc3 Antibody
Question
We are currently using anti-Morc3 antibody A06075-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on canine tissues as well?
D. Zhang
Verified customer
Asked: 2020-04-21
Answer
The anti-Morc3 antibody (A06075-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
Does anti-Morc3 antibody A06075-1 work for WB with trachea?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
According to the expression profile of trachea, MORC3 is highly expressed in trachea. So, it is likely that anti-Morc3 antibody A06075-1 will work for WB with trachea.
Boster Scientific Support
Answered: 2019-12-06
Question
Is a blocking peptide available for product anti-Morc3 antibody (A06075-1)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We do provide the blocking peptide for product anti-Morc3 antibody (A06075-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-07
Question
My question regarding product A06075-1, anti-Morc3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06075-1 anti-Morc3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-Morc3 antibody A06075-1 works with WB, what is the protocol used to produce the result images on the product page?
A. Taylor
Verified customer
Asked: 2015-12-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-02
