Anti-Morc3 Antibody

Product Name

Anti-Morc3 Antibody

View all MORC3 Antibodies

SKU/Catalog Number

A06075-1

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Morc3 Antibody (Catalog # A06075-1). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Morc3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06075-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Morc3 whole rabbit serum was prepared by repeated immunizations with a mouse Morc3 recombinant protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06075-1 is reactive to MORC3 in Mouse

Applications

A06075-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

107569 MW

Background of MORC3

The Morc (microrchidia) family of proteins are ATPases of the GHKL family. They have been implicated in transcriptional repression of genes and transposons, and higher order organization of DNA within the nucleus. Morc antibodies are ideal for researchers interested in Epigenetics, Cancer, and Cell cycle research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000-1:50,000
WB: 1:1000-1:5000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Morc3 using anti-Morc3 antibody (A06075-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Morc3 antigen affinity purified polyclonal antibody (Catalog # A06075-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Morc3.

Gene/Protein Information For MORC3 (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0136; MORC family CW-type zinc finger 3; MORC family CW-type zinc finger protein 3; nuclear matrix protein NXP2; NXP2; ZCW5; ZCWCC3; Zinc finger CW-type coiled-coil domain protein 3; zinc finger, CW type with coiled-coil domain 3; zinc finger, CW-type with coiled-coil domain 3 MORC3 NXP2, ZCW5, ZCWCC3 MORC family CW-type zinc finger 3 MORC family CW-type zinc finger protein 3|nuclear matrix protein 2|nuclear matrix protein NXP2|zinc finger CW-type coiled-coil domain protein 3|zinc finger, CW type with coiled-coil domain 3

For more info on MORC3, check out the MORC3 Infographic

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In this infographic, you will see the following information for MORC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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