Product Info Summary
SKU: | A09991 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-CARKL SHPK Antibody
SKU/Catalog Number
A09991
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CARKL SHPK Antibody (Catalog # A09991). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CARKL SHPK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09991)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human CARKL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09991 is reactive to SHPK in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
51491 MW
Background of CARKL
Acts as a modulator of macrophage activation through control of glucose metabolism By similarity.
Touchman J.W., Genome Res. 10:165-173(2000).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09991 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using CARKL antibody A09991.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using CARKL antibody A09991.
Protein Target Info & Infographic
Gene/Protein Information For SHPK (Source: Uniprot.org, NCBI)
Gene Name
SHPK
Full Name
Sedoheptulokinase
Weight
51491 MW
Superfamily
FGGY kinase family
Alternative Names
Sedoheptulokinase;SHK;2.7.1.14;Carbohydrate kinase-like protein;SHPK;CARKL; SHPK CARKL, SHK sedoheptulokinase sedoheptulokinase|carbohydrate kinase-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHPK, check out the SHPK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHPK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARKL SHPK Antibody (A09991)
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No publications found for A09991
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Customer Q&As
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4 Customer Q&As for Anti-CARKL SHPK Antibody
Question
We are currently using anti-CARKL antibody A09991 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-CARKL antibody (A09991) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
Question
I would like to test anti-CARKL antibody A09991 on human brain for research purposes, then I may be interested in using anti-CARKL antibody A09991 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
The products we sell, including anti-CARKL antibody A09991, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-21
Question
Will anti-CARKL antibody A09991 work for IHC with brain?
P. Bhatt
Verified customer
Asked: 2015-07-17
Answer
According to the expression profile of brain, SHPK is highly expressed in brain. So, it is likely that anti-CARKL antibody A09991 will work for IHC with brain.
Boster Scientific Support
Answered: 2015-07-17
Question
Would A09991 anti-CARKL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Li
Verified customer
Asked: 2014-12-22
Answer
It shows on the product datasheet, A09991 anti-CARKL antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-12-22