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Product Info Summary
| SKU: | A03783 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Mnk1 (Ab-385) MKNK1 Antibody
SKU/Catalog Number
A03783
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Mnk1 (Ab-385) MKNK1 Antibody (Catalog # A03783). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mnk1 (Ab-385) MKNK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03783)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized non-phosphopeptide derived from human Mnk1 around the phosphorylation site of threonine 385 (L-P-T(p)-P-Q).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03783 is reactive to MKNK1 in Human, Mouse
Reconstitution
Calculated molecular weight
51342 MW
Background of MNK1
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Fukunaga R., EMBO J. 16:1921-1933(1997).
Knauf U., Mol. Cell. Biol. 21:5500-5511(2001).
O'Loghlen A., Exp. Cell Res. 299:343-355(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03783 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Ab-385) antibody A03783.
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Western blot analysis of extracts from HepG2 cells (Lane 2), K562 cells (Lane 3) and Jurket cells (Lane 4), using Mnk1 (Ab-385) antiobdy A03783. The lane on the left is treated with systhesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For MKNK1 (Source: Uniprot.org, NCBI)
Gene Name
MKNK1
Full Name
MAP kinase-interacting serine/threonine-protein kinase 1
Weight
51342 MW
Superfamily
protein kinase superfamily
Alternative Names
MAP kinase-interacting serine/threonine-protein kinase 1;2.7.11.1;MAP kinase signal-integrating kinase 1;MAPK signal-integrating kinase 1;Mnk1;MKNK1;MNK1; MKNK1 MNK1 MAPK interacting serine/threonine kinase 1 MAP kinase-interacting serine/threonine-protein kinase 1|MAP kinase interacting serine/threonine kinase 1|MAP kinase signal-integrating kinase 1|MAPK signal-integrating kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKNK1, check out the MKNK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKNK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mnk1 (Ab-385) MKNK1 Antibody (A03783)
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No publications found for A03783
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Customer Q&As
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3 Customer Q&As for Anti-Mnk1 (Ab-385) MKNK1 Antibody
Question
Is there a BSA free version of anti-Mnk1 (Ab-385) antibody A03783 available?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mnk1 (Ab-385) antibody A03783 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-09
Question
I was wanting to use your anti-Mnk1 (Ab-385) antibody for WB for human body of pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human body of pancreas identification?
S. Mitchell
Verified customer
Asked: 2019-08-08
Answer
It shows on the product datasheet, A03783 anti-Mnk1 (Ab-385) antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human body of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
I have a question about product A03783, anti-Mnk1 (Ab-385) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Anderson
Verified customer
Asked: 2017-01-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03783 anti-Mnk1 (Ab-385) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-18
