Product Info Summary
SKU: | A14711-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MMTAG2 Antibody Picoband®
SKU/Catalog Number
A14711-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMTAG2 Antibody Picoband® catalog # A14711-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MMTAG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14711-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human C1orf35/MMTAG2 recombinant protein (Position: R27-E154). Human C1orf35/MMTAG2 shares 93.8% amino acid (aa) sequence identity with both mouse and rat C1orf35/MMTAG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14711-1 is reactive to MMTAG2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
29.3kDa
Background of Mmtag2
MMTAG2 4 isoforms of the human protein are produced by alternative splicing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14711-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human THP-1 whole cell, human MCF-7 whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
![a14711 1 mmtag2 primary antibodies wb testing 1 a14711 1 mmtag2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a14711-1-mmtag2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of C1orf35/MMTAG2 using anti-C1orf35/MMTAG2 antibody (A14711-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf35/MMTAG2 antigen affinity purified polyclonal antibody (Catalog # A14711-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1orf35/MMTAG2 at approximately 35 kDa. The expected band size for C1orf35/MMTAG2 is at 29 kDa.
![a14711 1 mmtag2 primary antibodies fcm testing 2 a14711 1 mmtag2 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a14711-1-mmtag2-primary-antibodies-fcm-testing-2.png)
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-C1orf35/MMTAG2 antibody (A14711-1).
Overlay histogram showing MCF-7 cells stained with A14711-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf35/MMTAG2 Antibody (A14711-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MMTAG2 (Source: Uniprot.org, NCBI)
Gene Name
MMTAG2
Full Name
Multiple myeloma tumor-associated protein 2 homolog
Weight
29.3kDa
Alternative Names
Multiple myeloma tumor-associated protein 2 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMTAG2, check out the MMTAG2 Infographic
![MMTAG2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMTAG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMTAG2 Antibody Picoband® (A14711-1)
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