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Product Info Summary
| SKU: | A03087-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MLX Antibody Picoband®
SKU/Catalog Number
A03087-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MLX Antibody Picoband® catalog # A03087-3. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MLX Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03087-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MLX recombinant protein (Position: R33-Y298).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03087-3 is reactive to MLX in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
64406 MW
Background of MLX
Max-like protein X is a protein that in humans is encoded by the MLX gene. The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03087-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human
ELISA, 0.1-0.5 μg/ml/ml, Human
Positive Control
WB: human HepG2 whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MLX using anti-MLX antibody (A03087-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 33 kDa. The expected band size for MLX is at 30 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MLX (Source: Uniprot.org, NCBI)
Gene Name
MLX
Full Name
Max-like protein X
Weight
64406 MW
Alternative Names
Aquaporin-5; AQP-5; AQP5 MLX MAD7, MXD7, TCFL4, TF4, bHLHd13 MAX dimerization protein MLX max-like protein X|BigMax protein|MAX-like bHLHZIP protein|MLX, MAX dimerization protein|class D basic helix-loop-helix protein 13|transcription factor-like protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MLX, check out the MLX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MLX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MLX Antibody Picoband® (A03087-3)
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