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Product Info Summary
| SKU: | A05385-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MLN64/STARD3 Antibody Picoband®
SKU/Catalog Number
A05385-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MLN64/STARD3 Antibody Picoband® catalog # A05385-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MLN64/STARD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05385-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MLN64/STARD3 recombinant protein (Position: K3-E429).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05385-1 is reactive to STARD3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
39411 MW
Background of STARD3
StAR related lipid transfer domain containing 3 (STARD3) is a protein that in humans is encoded by the STARD3 gene. This gene encodes a member of a subfamily of lipid trafficking proteins that are characterized by a C-terminal steroidogenic acute regulatory domain and an N-terminal metastatic lymph node 64 domain. The encoded protein localizes to the membranes of late endosomes and may be involved in exporting cholesterol. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05385-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MLN64/STARD3 using anti-MLN64/STARD3 antibody (A05385-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLN64/STARD3 antigen affinity purified polyclonal antibody (Catalog # A05385-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLN64/STARD3 at approximately 51 kDa. The expected band size for MLN64/STARD3 is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For STARD3 (Source: Uniprot.org, NCBI)
Gene Name
STARD3
Full Name
StAR-related lipid transfer protein 3
Weight
39411 MW
Superfamily
STARD3 family
Alternative Names
Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097 STARD3 CAB1, MLN64, es64 StAR related lipid transfer domain containing 3 stAR-related lipid transfer protein 3|MLN 64|START domain-containing protein 3|StAR-related lipid transfer (START) domain containing 3|metastatic lymph node gene 64 protein|metastatic lymph node protein 64|steroidogenic acute regulatory protein related
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STARD3, check out the STARD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STARD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MLN64/STARD3 Antibody Picoband® (A05385-1)
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