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Product Info Summary
| SKU: | A02916 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MKK3 (Ab-189) MAP2K3 Antibody
SKU/Catalog Number
A02916
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MKK3 (Ab-189) MAP2K3 Antibody (Catalog # A02916). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MKK3 (Ab-189) MAP2K3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02916)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02916 is reactive to MAP2K3 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23593 MW
Background of MKK3/MEK3
MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02916 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (A02916).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (Catalog # A02916) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAP2K3.
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Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Ab-189) Antibody #A02916.
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Figure 2. IHC analysis of MAP2K3 using anti-MAP2K3 antibody (A02916).
MAP2K3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K3 Antibody (A02916) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K3
Full Name
Dual specificity mitogen-activated protein kinase kinase 3
Weight
23593 MW
Superfamily
protein kinase superfamily
Alternative Names
Ras-related protein Rab-6A;Rab-6;RAB6A;RAB6; MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2 mitogen-activated protein kinase kinase 3 dual specificity mitogen-activated protein kinase kinase 3|MAP kinase kinase 3|MAPK/ERK kinase 3|MAPKK 3|MEK 3|SAPK kinase 2|stress-activated protein kinase kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K3, check out the MAP2K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MKK3 (Ab-189) MAP2K3 Antibody (A02916)
Hello CJ!
A02916 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu G, Zhang Y, Wei J, Jia W, Ge Z, Zhang Z, Liu X. Bmc Cancer. 2013 Oct 10;13:469. Doi: 10.1186/1471-2407-13-469. Microrna-21 Promotes Hepatocellular Carcinoma Hepg2 Cell Proliferation Through Repression Of Mitogen-Activated Protein Kinase-Kinase 3.
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