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Product Info Summary
| SKU: | A32122 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MINA RIOX2 Antibody
SKU/Catalog Number
A32122
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MINA RIOX2 Antibody (Catalog # A32122). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MINA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MINA RIOX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32122)
Host
Rabbit
Contents
MINA Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MINA antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MINA. The immunogen is located within amino acids 380 - 430 of MINA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
It is predicted to not cross-react with other members of the cavin family.
Reactive Species
A32122 is reactive to MINA in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
52800 MW
Background of RIOX2
MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32122 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of MINA in mouse heart tissue with MINA antibody at 5 μg/mL.
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Immunofluorescence of MINA in mouse heart tissue with MINA antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MINA (Source: Uniprot.org, NCBI)
Gene Name
MINA
Full Name
Ribosomal oxygenase 2
Weight
52800 MW
Superfamily
ROX family
Alternative Names
ROX, MDIG, NO52, MINA53, Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, 60S ribosomal protein L27a histidine hydroxylase, ROX RIOX2 JMJD10, MDIG, MINA, MINA53, NO52, ROX ribosomal oxygenase 2 ribosomal oxygenase 2|60S ribosomal protein L27a histidine hydroxylase|MYC induced nuclear |MYC-induced nuclear 53|bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA|histone lysine demethylase MINA|mineral dust induced gene protein|myc-induced nuclear , 53 kDa|nucleolar protein 52|ribosomal oxygenase MINA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MINA, check out the MINA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MINA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MINA RIOX2 Antibody (A32122)
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