Product Info Summary
SKU: | A06887-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MIEF1 Antibody Picoband®
SKU/Catalog Number
A06887-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIEF1 Antibody Picoband® catalog # A06887-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIEF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06887-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MIEF1 recombinant protein (Position: S189-T463).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06887-1 is reactive to MIEF1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
87148 MW
Background of MIEF1
Mitochondrial dynamic protein MID51 (MID51) also known as mitochondrial elongation factor 1 (MIEF1) or Smith-Magenis syndrome chromosome region candidate gene 7 protein-like (SMCR7L) is a protein that in humans is encoded by the SMCR7L gene. The SMCR7L gene codes for a protein that has been called MiD51/MIEF1 and shown to regulate mitochondrial fission by interacting with the proteins Drp1 and FIS1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06887-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human HT1080 whole cell, human Jurkat whole cell, human K562 whole cell, human HL-60 whole cell, human HEK293 whole cell, human U-937 whole cell, human HepG2 whole cell, rat heart tissue, rat kidney tissue, mouse kidney tissue
IHC: human renal clear cell carcinoma tissue, human rectal cancer tissue
ICC/IF: U20S cell
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MIEF1 using anti-MIEF1 antibody (A06887-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HT1080 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human U-937 whole cell lysates,
Lane 7: human HepG2 whole cell lysates,
Lane 8: rat heart tissue lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIEF1 antigen affinity purified polyclonal antibody (Catalog # A06887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIEF1 at approximately 51KD. The expected band size for MIEF1 is at 51KD.
Click image to see more details
Figure 2. IHC analysis of MIEF1 using anti-MIEF1 antibody (A06887-1).
MIEF1 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MIEF1 Antibody (A06887-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MIEF1 using anti-MIEF1 antibody (A06887-1).
MIEF1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MIEF1 Antibody (A06887-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of MIEF1 using anti-MIEF1 antibody (A06887-1).
MIEF1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MIEF1 Antibody (A06887-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of SiHa cells using anti-MIEF1 antibody (A06887-1).
Overlay histogram showing SiHa cells stained with A06887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIEF1 Antibody (A06887-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MIEF1 (Source: Uniprot.org, NCBI)
Gene Name
MIEF1
Full Name
MIEF1 upstream open reading frame protein
Weight
87148 MW
Superfamily
complex I LYR family
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 28;ADAM 28;3.4.24.-;Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II;eMDC II;Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L;MDC-L;ADAM28;ADAM23, MDCL; MIEF1 AltMIEF1, HSU79252, MID51-MP, SMCR7L, dJ1104E15.3, MIEF1 mitochondrial elongation factor 1 mitochondrial dynamics protein MID51|MIEF1 microprotein|SMCR7-like protein|Smith-Magenis syndrome chromosome region, candidate 7-like|alternative MIEF1 protein|mitochondrial dynamic protein MID51|mitochondrial dynamic protein of 51 kDa|mitochondrial dynamics protein of 51 kDa|smith-Magenis syndrome chromosomal region candidate gene 7 protein-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MIEF1, check out the MIEF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MIEF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MIEF1 Antibody Picoband® (A06887-1)
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