Product Info Summary
SKU: | A01012-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MGMT Antibody
SKU/Catalog Number
A01012-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MGMT Antibody catalog # A01012-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MGMT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01012-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MGMT. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01012-1 is reactive to MGMT in Human, Rat
Reconstitution
Calculated molecular weight
21646 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01012-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-MGMT Antibody A01012-1
Western Blot (WB) analysis of specific cells using MGMT polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-MGMT Antibody A01012-1
Western Blot (WB) analysis of Jurkat cells using MGMT polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MGMT (Source: Uniprot.org, NCBI)
Gene Name
MGMT
Full Name
Methylated-DNA--protein-cysteine methyltransferase
Weight
21646 MW
Superfamily
MGMT family
Alternative Names
Methylated-DNA--protein-cysteine methyltransferase;2.1.1.63;6-O-methylguanine-DNA methyltransferase;MGMT;O-6-methylguanine-DNA-alkyltransferase;MGMT; MGMT O-6-methylguanine-DNA methyltransferase methylated-DNA--protein-cysteine methyltransferase|6-O-methylguanine-DNA methyltransferase|O-6-methylguanine-DNA-alkyltransferase|O6-methylguanine-DNA methyltransferase|methylguanine-DNA methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MGMT, check out the MGMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MGMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MGMT Antibody (A01012-1)
Hello CJ!
A01012-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Minea RO,Duc TC,Swenson SD,Cho HY,Huang M,Hartman H,Hofman FM,Schönthal AH,Chen TC.Developing a clinically relevant radiosensitizer for temozolomide-resistant gliomas.PLoS One.2020 Sep 3;15(9):e0238238.doi:10.1371/journal.pone.0238238.PMID:32881880;PMCID:
Species: Human,Mouse
A01012-1 usage in article: APP:WB, SAMPLE:HUMAN GB CELL LINES, DILUTION:NA
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