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Product Info Summary
| SKU: | PA1377-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MEK3/MAP2K3 Antibody Picoband®
SKU/Catalog Number
PA1377-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEK3/MAP2K3 Antibody catalog # PA1377-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK3/MAP2K3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1377-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MEK3, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1377-1 is reactive to MAP2K3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39318 MW
Background of MKK3/MEK3
Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al. (1997) localized the MAP2K3 gene to 17q11.2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1377-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Skeletal Muscle Tissue, Rat Kidney Tissue, Rat Spleen Tissue, HELA Whole Cell, A549 Whole Cell, JURKAT Whole Cell, CEM Whole Cell
Validation Images & Assay Conditions
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Anti-MEK3 antibody, PA1377-1, All Western blotting
All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: Rat Spleen Tissue Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: CEM Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Protein Target Info & Infographic
Gene/Protein Information For MAP2K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K3
Full Name
Dual specificity mitogen-activated protein kinase kinase 3
Weight
39318 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 3;MAP kinase kinase 3;MAPKK 3;2.7.12.2;MAPK/ERK kinase 3;MEK 3;Stress-activated protein kinase kinase 2;SAPK kinase 2;SAPKK-2;SAPKK2;MAP2K3;MEK3, MKK3, PRKMK3, SKK2; MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2 mitogen-activated protein kinase kinase 3 dual specificity mitogen-activated protein kinase kinase 3|MAP kinase kinase 3|MAPK/ERK kinase 3|MAPKK 3|MEK 3|SAPK kinase 2|stress-activated protein kinase kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K3, check out the MAP2K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK3/MAP2K3 Antibody Picoband® (PA1377-1)
Hello CJ!
PA1377-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu G, Zhang Y, Wei J, Jia W, Ge Z, Zhang Z, Liu X. Bmc Cancer. 2013 Oct 10;13:469. Doi: 10.1186/1471-2407-13-469. Microrna-21 Promotes Hepatocellular Carcinoma Hepg2 Cell Proliferation Through Repression Of Mitogen-Activated Protein Kinase-Kinase 3.
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MEK3/MAP2K3 Antibody Picoband®
Question
Do you have a BSA free version of anti-MEK3/MAP2K3 antibody PA1377-1 available?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MEK3/MAP2K3 antibody PA1377-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-08
Question
See attached the WB image, lot number and protocol we used for muscle using anti-MEK3/MAP2K3 antibody PA1377-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
My question regarding product PA1377-1, anti-MEK3/MAP2K3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1377-1 anti-MEK3/MAP2K3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-20
Question
Would PA1377-1 anti-MEK3/MAP2K3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
You can see on the product datasheet, PA1377-1 anti-MEK3/MAP2K3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-05
Question
Would anti-MEK3/MAP2K3 antibody PA1377-1 work for WB with muscle?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
According to the expression profile of muscle, MAP2K3 is highly expressed in muscle. So, it is likely that anti-MEK3/MAP2K3 antibody PA1377-1 will work for WB with muscle.
Boster Scientific Support
Answered: 2017-12-12
Question
Would anti-MEK3/MAP2K3 antibody PA1377-1 work on pig WB with leukocyte?
C. Jackson
Verified customer
Asked: 2017-07-14
Answer
Our lab technicians have not validated anti-MEK3/MAP2K3 antibody PA1377-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MEK3/MAP2K3 antibody PA1377-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-14
Question
Is this PA1377-1 anti-MEK3/MAP2K3 antibody reactive to the isotypes of MAP2K3?
F. Evans
Verified customer
Asked: 2013-07-17
Answer
The immunogen of PA1377-1 anti-MEK3/MAP2K3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human MEK3(42-58aa RNLDSRTFITIGDRNFE), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-17
