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Product Info Summary
| SKU: | P01131 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-MEF2C (Ser222) Antibody
SKU/Catalog Number
P01131
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-MEF2C (Ser222) Antibody (Catalog # P01131). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-MEF2C (Ser222) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01131)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser222 of human MEF2C, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01131 is reactive to MEF2C in Human
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
51278 MW
Background of MEF2C
MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C, is one of 4 MEF2 (myocyte enhancer factor 2) transcription factors that encode proteins for development of skeletal muscle and brain proliferation and differentiation (McDermott et al, 1993), along with regulating stress-response during cardiac hypertrophy in mammals (Wu et al, 2015). Phosphorylation of MEF2C at serine 59 has been shown to be negatively regulated by integrin-linked kinase (ILK) (Dong et al, 2015). Recently, high MEF2C expression has been associated with a subset of acute myeloid leukemia (AML) patients with adverse-risk disease features and poor outcomes (Laszlo et al, 2015). Phosphorylation of MEF2C at serine 222 may play a key role in MEF2C signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01131 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ((gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For MEF2C (Source: Uniprot.org, NCBI)
Gene Name
MEF2C
Full Name
Myocyte-specific enhancer factor 2C
Weight
51278 MW
Superfamily
MEF2 family
Alternative Names
C5DELq14.3 antibody, DEL5q14.3 antibody, MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody, MADS box transcription enhancer factor 2 polypeptide C antibody, MEF2C antibody, MEF2C_HUMAN antibody, Myocyte enhancer factor 2C antibody, Myocyte specific enhancer factor 2C antibody, Myocyte-specific enhancer factor 2C antibody, OTTHUMP00000222409 antibody, Similar to MADS box transcription enhancer factor 2 polypeptide C antibody MEF2C C5DELq14.3, DEL5q14.3 myocyte enhancer factor 2C myocyte-specific enhancer factor 2C|MADS box transcription enhancer factor 2, polypeptide C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MEF2C, check out the MEF2C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MEF2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MEF2C (Ser222) Antibody (P01131)
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No publications found for P01131
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-MEF2C (Ser222) Antibody
Question
I was wanting to use your anti-Phospho-MEF2C (Ser222) antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
As indicated on the product datasheet, P01131 anti-Phospho-MEF2C (Ser222) antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-28
Question
I have a question about product P01131, anti-Phospho-MEF2C (Ser222) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P01131 anti-Phospho-MEF2C (Ser222) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-26
Question
Would anti-Phospho-MEF2C (Ser222) antibody P01131 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
According to the expression profile of erythroleukemia, MEF2C is highly expressed in erythroleukemia. So, it is likely that anti-Phospho-MEF2C (Ser222) antibody P01131 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2019-11-25
Question
We were happy with the WB result of your anti-Phospho-MEF2C (Ser222) antibody. However we have been able to see positive staining in skeletal muscle nucleus. using this antibody. Is that expected? Could you tell me where is MEF2C supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
According to literature, skeletal muscle does express MEF2C. Generally MEF2C expresses in nucleus. Regarding which tissues have MEF2C expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, and Muscle, Pubmed ID: 7679508
Skeletal muscle, Pubmed ID: 8455629, 17974005
Boster Scientific Support
Answered: 2019-10-18
Question
See attached the WB image, lot number and protocol we used for erythroleukemia using anti-Phospho-MEF2C (Ser222) antibody P01131. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-Phospho-MEF2C (Ser222) antibody P01131 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The anti-Phospho-MEF2C (Ser222) antibody (P01131) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-21
Question
I see that the anti-Phospho-MEF2C (Ser222) antibody P01131 works with WB, what is the protocol used to produce the result images on the product page?
A. Yang
Verified customer
Asked: 2018-11-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-08
Question
Does P01131 anti-Phospho-MEF2C (Ser222) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
It shows on the product datasheet, P01131 anti-Phospho-MEF2C (Ser222) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-12
Question
Do you have a BSA free version of anti-Phospho-MEF2C (Ser222) antibody P01131 available?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-MEF2C (Ser222) antibody P01131 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-06
Question
Is this P01131 anti-Phospho-MEF2C (Ser222) antibody reactive to the isotypes of MEF2C?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
The immunogen of P01131 anti-Phospho-MEF2C (Ser222) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser222 of the human MEF2C protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Phospho-MEF2C (Ser222) antibody P01131. Let me know if you need anything else.
D. Anderson
Verified customer
Asked: 2016-05-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-20
Question
My question regards to test anti-Phospho-MEF2C (Ser222) antibody P01131 on human erythroleukemia for research purposes, then I may be interested in using anti-Phospho-MEF2C (Ser222) antibody P01131 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Li
Verified customer
Asked: 2016-03-23
Answer
The products we sell, including anti-Phospho-MEF2C (Ser222) antibody P01131, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-23
Question
We have seen staining in human erythroleukemia. Are there any suggestions? Is anti-Phospho-MEF2C (Ser222) antibody supposed to stain erythroleukemia positively?
C. Zhao
Verified customer
Asked: 2015-12-22
Answer
Based on literature erythroleukemia does express MEF2C. Based on Uniprot.org, MEF2C is expressed in middle temporal gyrus, fetal brain muscle, skeletal muscle, erythroleukemia, among other tissues. Regarding which tissues have MEF2C expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, and Muscle, Pubmed ID: 7679508
Skeletal muscle, Pubmed ID: 8455629, 17974005
Boster Scientific Support
Answered: 2015-12-22
Question
Is a blocking peptide available for product anti-Phospho-MEF2C (Ser222) antibody (P01131)?
J. Zhao
Verified customer
Asked: 2014-12-09
Answer
We do provide the blocking peptide for product anti-Phospho-MEF2C (Ser222) antibody (P01131). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-09
Question
Will anti-Phospho-MEF2C (Ser222) antibody P01131 work on feline WB with fetal brain muscle?
C. Jones
Verified customer
Asked: 2013-08-05
Answer
Our lab technicians have not validated anti-Phospho-MEF2C (Ser222) antibody P01131 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-MEF2C (Ser222) antibody P01131 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline fetal brain muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-05
