Anti-MED24/Trap100 Antibody

Product Name

Anti-MED24/Trap100 Antibody

View all MED24 Antibodies

SKU/Catalog Number

A05987

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MED24/Trap100 Antibody (Catalog # A05987). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED24/Trap100 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05987)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MED24.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05987 is reactive to MED24 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

110305 MW

Background of MED24

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
Rachez C., Genes Dev. 12:1787-1800(1998).
Nagase T., DNA Res. 2:167-174(1995).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A05987 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MED24 (Source: Uniprot.org, NCBI)

Alternative Names

Mediator of RNA polymerase II transcription subunit 24;Activator-recruited cofactor 100 kDa component;ARC100;Cofactor required for Sp1 transcriptional activation subunit 4;CRSP complex subunit 4;Mediator complex subunit 24;Thyroid hormone receptor-associated protein 4;Thyroid hormone receptor-associated protein complex 100 kDa component;Trap100;hTRAP100;Vitamin D3 receptor-interacting protein complex 100 kDa component;DRIP100;MED24;ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100; MED24 ARC100, CRSP100, CRSP4, DRIP100, MED5, THRAP4, TRAP100 mediator complex subunit 24 mediator of RNA polymerase II transcription subunit 24|CRSP complex subunit 4|activator-recruited cofactor 100 kDa component|cofactor required for Sp1 transcriptional activation subunit 4|cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa|mediator of RNA polymerase II transcription, subunit 24 homolog|thyroid hormone receptor-associated protein 4|thyroid hormone receptor-associated protein complex 100 kDa component|vitamin D3 receptor-interacting protein complex 100 kDa component|vitamin D3 receptor-interacting protein complex component DRIP100

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis