Anti-MED21/Srb7 Antibody

Product Name

Anti-MED21/Srb7 Antibody

View all MED21 Antibodies

SKU/Catalog Number

A10271

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MED21/Srb7 Antibody (Catalog # A10271). Tested in IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED21/Srb7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10271)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MED21.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10271 is reactive to MED21 in Human, Mouse

Reconstitution

Calculated molecular weight

15564 MW

Background of MED21

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Chao D.M., Nature 380:82-85(1996).
Maldonado E., Nature 381:86-89(1996).
Oelgeschlaeger T., Mol. Cell 1:925-931(1998).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10271 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For MED21 (Source: Uniprot.org, NCBI)

Alternative Names

Mediator of RNA polymerase II transcription subunit 21;Mediator complex subunit 21;RNA polymerase II holoenzyme component SRB7;RNAPII complex component SRB7;hSrb7;MED21;SRB7, SURB7; MED21 SRB7, SURB7, hSrb7 mediator complex subunit 21 mediator of RNA polymerase II transcription subunit 21|RNA polymerase II holoenzyme component SRB7|RNAPII complex component SRB7|SRB7 suppressor of RNA polymerase B homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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