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Product Info Summary
| SKU: | M03449 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ME1 Antibody Picoband® (monoclonal, 5E5F7)
SKU/Catalog Number
M03449
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ME1 Antibody Picoband® (monoclonal, 5E5F7) catalog # M03449. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ME1 Antibody Picoband® (monoclonal, 5E5F7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03449)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
5E5F7
Isotype
Mouse IgG1
Immunogen
E.coli-derived human ME1 recombinant protein (Position: M1-Q572).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03449 is reactive to ME1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
64 kDa
Calculated molecular weight
64.15kDa
Background of ME1
NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03449 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Positive Control
WB: human A549 whole cell, human Hela whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue
IHC: human liver cancer tissue, human spleen tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ME1 using anti-ME1 antibody (M03449).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ME1 antigen affinity purified monoclonal antibody (Catalog # M03449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.
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Figure 2. IHC analysis of ME1 using anti-ME1 antibody (M03449).
ME1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 3. IHC analysis of ME1 using anti-ME1 antibody (M03449).
ME1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ME1 using anti-ME1 antibody (M03449).
ME1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ME1 (Source: Uniprot.org, NCBI)
Gene Name
ME1
Full Name
NADP-dependent malic enzyme
Weight
64.15kDa
Superfamily
malic enzymes family
Alternative Names
Survival motor neuron protein; Component of gems 1; Gemin-1; SMN1; SMN, SMNT; SMN2; SMNC ME1 HUMNDME, MES malic enzyme 1 NADP-dependent malic enzyme|Malic enzyme, cytoplasmic|NADP-ME|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))|malic enzyme 1, NADP(+)-dependent, cytosolic|malic enzyme 1, soluble|pyruvic-malic carboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ME1, check out the ME1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ME1 Antibody Picoband® (monoclonal, 5E5F7) (M03449)
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