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Product Info Summary
| SKU: | A04262-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MDH1 Antibody (C-term)
SKU/Catalog Number
A04262-1
Size
80 µl
Description
Boster Bio Anti-MDH1 Antibody (C-term) (Catalog # A04262-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MDH1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04262-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04262-1 is reactive to MDH1 in Human
Reconstitution
Calculated molecular weight
36426 Da
Background of MDH1
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04262-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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MDH1 Antibody (C-term) western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).This demonstrates the MDH1 antibody detected the MDH1 protein (arrow).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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MDH1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For MDH1 (Source: Uniprot.org, NCBI)
Gene Name
MDH1
Full Name
Malate dehydrogenase, cytoplasmic
Weight
36426 Da
Superfamily
LDH/MDH superfamily
Alternative Names
Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductase, MDH1, MDHA MDH1 DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2 malate dehydrogenase 1 malate dehydrogenase, cytoplasmic|malate dehydrogenase, peroxisomal|cytosolic malate dehydrogenase|diiodophenylpyruvate reductase|epididymis secretory protein Li 32|malate dehydrogenase 1, NAD (soluble)|soluble malate dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDH1, check out the MDH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDH1 Antibody (C-term) (A04262-1)
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