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Product Info Summary
| SKU: | A17113-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MCEMP1 Antibody Picoband®
SKU/Catalog Number
A17113-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCEMP1 Antibody Picoband® catalog # A17113-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MCEMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17113-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MCEMP1 recombinant protein (Position: M1-Q183). Human MCEMP1 shares 43.5% amino acid (aa) sequence identity with mouse MCEMP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A17113-1 is reactive to MCEMP1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
20.649kDa
Background of Mcemp1
This gene encodes a single-pass transmembrane protein. Based on its expression pattern, it is speculated to be involved in regulating mast cell differentiation or immune responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17113-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell, human HEL whole cell, human K562 whole cell, rat liver tissue, rat lung tissue, mouse liver tissue, mouse Hepa1-6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MCEMP1 using anti-MCEMP1 antibody (A17113-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCEMP1 antigen affinity purified polyclonal antibody (Catalog # A17113-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCEMP1 at approximately 28 kDa. The expected band size for MCEMP1 is at 21 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MCEMP1 (Source: Uniprot.org, NCBI)
Gene Name
MCEMP1
Full Name
Mast cell-expressed membrane protein 1
Weight
20.649kDa
Alternative Names
C19orf59; chromosome 19 open reading frame 59; mast cell-expressed membrane protein 1; MCEMP1; MGC132456
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCEMP1, check out the MCEMP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCEMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCEMP1 Antibody Picoband® (A17113-1)
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