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Product Info Summary
| SKU: | A04529-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MATE-1/SLC47A1 Antibody Picoband®
SKU/Catalog Number
A04529-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MATE-1/SLC47A1 Antibody Picoband® catalog # A04529-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MATE-1/SLC47A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04529-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MATE-1/SLC47A1 recombinant protein (Position: M1-D508).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04529-2 is reactive to SLC47A1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
61.922kDa
Background of MATE1
Multidrug and toxin extrusion protein 1 (MATE1), also known as solute carrier family 47 member 1, is a protein that in humans is encoded by the SLC47A1 gene. This gene is located within the Smith-Magenis syndrome region on chromosome 17. It encodes a protein of unknown function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04529-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MATE-1/SLC47A1 using anti-MATE-1/SLC47A1 antibody (A04529-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATE-1/SLC47A1 antigen affinity purified polyclonal antibody (Catalog # A04529-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATE-1/SLC47A1 at approximately 32 kDa. The expected band size for MATE-1/SLC47A1 is at 62 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SLC47A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC47A1
Full Name
Multidrug and toxin extrusion protein 1
Weight
61.922kDa
Superfamily
multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
Alternative Names
Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3 SLC47A1 MATE1 solute carrier family 47 member 1 multidrug and toxin extrusion protein 1|MATE-1|hMATE-1|multidrug and toxin extrusion 1|solute carrier family 47 (multidrug and toxin extrusion), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC47A1, check out the SLC47A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC47A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MATE-1/SLC47A1 Antibody Picoband® (A04529-2)
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