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Product Info Summary
| SKU: | M08039 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody
SKU/Catalog Number
M08039
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody catalog # M08039. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08039)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FHH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Mast Cell Tryptase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08039 is reactive to TPSAB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
30515 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08039 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: Human tonsil
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of Mast Cell Tryptase expression in Human tonsil lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Mast Cell Tryptase Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TPSAB1 (Source: Uniprot.org, NCBI)
Gene Name
TPSAB1
Full Name
Tryptase alpha/beta-1
Weight
30515 MW
Superfamily
peptidase S1 family
Alternative Names
Tryptase alpha/beta-1;Tryptase-1;3.4.21.59;Tryptase I;Tryptase alpha-1;TPSAB1;TPS1, TPS2, TPSB1; TPSAB1 TPS1, TPS2, TPSB1, TPSB2, Tryptase-2 tryptase alpha/beta 1 tryptase alpha/beta-1|Tryptase II|Tryptase beta-2|epididymis secretory sperm binding protein|mast cell alpha II tryptase|mast cell beta I tryptase|tryptase alpha II|tryptase alpha-1|tryptase beta I|tryptase beta-1|tryptase-1|tryptase-I|tryptase-III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPSAB1, check out the TPSAB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPSAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody (M08039)
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Customer Q&As
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5 Customer Q&As for Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-Mast Cell Tryptase Rabbit Monoclonal antibody M08039 available?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mast Cell Tryptase Rabbit Monoclonal antibody M08039 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-02
Question
I have a question about product M08039, anti-Mast Cell Tryptase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08039 anti-Mast Cell Tryptase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-25
Question
Is this M08039 anti-Mast Cell Tryptase Rabbit Monoclonal antibody reactive to the isotypes of TPSAB1?
M. Banerjee
Verified customer
Asked: 2019-01-15
Answer
The immunogen of M08039 anti-Mast Cell Tryptase Rabbit Monoclonal antibody is A synthesized peptide derived from human Mast Cell Tryptase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-15
Question
Does M08039 anti-Mast Cell Tryptase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
It shows on the product datasheet, M08039 anti-Mast Cell Tryptase Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-20
Question
I was wanting to use your anti-Mast Cell Tryptase Rabbit Monoclonal antibody for IP for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
You can see on the product datasheet, M08039 anti-Mast Cell Tryptase Rabbit Monoclonal antibody has been tested for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-22
