Product Info Summary
SKU: | M03409 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-MASPIN Antibody Picoband™ (monoclonal, 7G4E1)
View all Serpin B5/Maspin Antibodies
SKU/Catalog Number
M03409
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MASPIN Antibody Picoband™ (monoclonal, 7G4E1) catalog # M03409. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MASPIN Antibody Picoband™ (monoclonal, 7G4E1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03409)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
7G4E1
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03409 is reactive to SERPINB5 in Human
Applications
M03409 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
Background of Serpin B5/Maspin
SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MASPIN using anti-MASPIN antibody (M03409).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MASPIN antigen affinity purified monoclonal antibody (Catalog # M03409) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42 kDa. The expected band size for MASPIN is at 42 kDa.
Click image to see more details
Figure 2. IHC analysis of MASPIN using anti-MASPIN antibody (M03409).
MASPIN was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MASPIN Antibody (M03409) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MASPIN using anti-MASPIN antibody (M03409).
MASPIN was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MASPIN Antibody (M03409) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of SiHa cells using anti-MASPIN antibody (M03409).
Overlay histogram showing SiHa cells stained with M03409 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MASPIN Antibody (M03409, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SERPINB5 (Source: Uniprot.org, NCBI)
Gene Name
SERPINB5
Full Name
Serpin B5
Weight
Superfamily
serpin family
Alternative Names
Maspin; Peptidase inhibitor 5; PI5; PI-5; PI5protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin peptidase inhibitor, clade B (ovalbumin), member 5 SERPINB5 PI5, maspin serpin family B member 5 serpin B5|PI-5|peptidase inhibitor 5|protease inhibitor 5 (maspin)|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5|serpin peptidase inhibitor, clade B (ovalbumin), member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINB5, check out the SERPINB5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MASPIN Antibody Picoband™ (monoclonal, 7G4E1) (M03409)
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