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Product Info Summary
| SKU: | M04133 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody
View all MARCKS like protein Antibodies
SKU/Catalog Number
M04133
Size
100ul
Description
Boster Bio Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody catalog # M04133. Tested in IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04133)
Host
Rabbit
Contents
Supplied as an aliquot of serum plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full length recombinant human MARCKS purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04133 is reactive to Marcksl1 in Human
Reconstitution
Calculated molecular weight
20165 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04133 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1:10,000-1:20,000. IF/ICC and IHC: 1:500-1:1,000.
Validation Images & Assay Conditions
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Western blot analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS, M04133, dilution 1:1,000, in green and mouse mAb to GAPDH, dilution 1:5,000 in red: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6 cells. The strong band at ~80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [5], but no reactivity is seen on rodent cells. GAPDH antibody used as a loading control revealing a single band at ~37kDa in all preparations.
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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to MARCKS, M04133, dilution 1:1,000 in red, and costained with mouse mAb to -tubulin, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The M04133 antibody recognizes protein localized in the plasma membrane and cytoplasm, while the -tubulin antibody stains the network of cytoplasmic microtubules.
Protein Target Info & Infographic
Gene/Protein Information For Marcksl1 (Source: Uniprot.org, NCBI)
Gene Name
Marcksl1
Full Name
MARCKS-related protein
Weight
20165 MW
Superfamily
MARCKS family
Alternative Names
MARCKS-related protein;Brain protein F52;MARCKS-like protein 1;Macrophage myristoylated alanine-rich C kinase substrate;Mac-MARCKS;MacMARCKS;Marcksl1;Mlp, Mrp; Marcksl1|AL022768, AW215397, AW536807, D4Bc1, F52, M, Mac, MacMA, MacMARCKS, Macs, Macs2, Macs3, Mlp, Mrp|MARCKS-like 1|MARCKS-related protein|MARCKS-like protein 1|brain protein F52|mac-MARCKS|macrophage myristoylated alanine-rich C kinase substrate
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Marcksl1, check out the Marcksl1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Marcksl1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody (M04133)
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Customer Q&As
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1 Customer Q&As for Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody
Question
We are currently using anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) antibody M04133 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
The anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) antibody (M04133) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-06
