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Product Info Summary
| SKU: | A12206 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-MAK10 NAA35 Antibody
SKU/Catalog Number
A12206
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MAK10 NAA35 Antibody (Catalog # A12206). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MAK10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MAK10 NAA35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12206)
Host
Rabbit
Contents
MAK10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MAK10 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human MAK10. The immunogen is located within amino acids 190 - 240 of MAK10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
UHRF1BP1 antibody is predicted to not cross-react with UHRF1BP1L
Reactive Species
A12206 is reactive to MAK10 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
83639 MW
Background of MAK10
The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12206 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MAK10 antibody can be used for detection of MAK10 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MAK10 in rat heart tissue lysate with MAK10 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
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Immunohistochemistry of MAK10 in human heart tissue with MAK10 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MAK10 (Source: Uniprot.org, NCBI)
Gene Name
MAK10
Full Name
N-alpha-acetyltransferase 35, NatC auxiliary subunit
Weight
83639 MW
Superfamily
MAK10 family
Alternative Names
EGAP, MAK10, MAK10P, bA379P1.1, EGAP, N-alpha-acetyltransferase 35, NatC auxiliary subunit, Embryonic growth-associated protein homolog NAA35 EGAP, MAK10, MAK10P, bA379P1.1 N-alpha-acetyltransferase 35, NatC auxiliary subunit N-alpha-acetyltransferase 35, NatC auxiliary subunit|MAK10 homolog, amino-acid N-acetyltransferase subunit|corneal wound healing-related protein|embryonic growth-associated protein homolog|protein MAK10 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAK10, check out the MAK10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAK10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAK10 NAA35 Antibody (A12206)
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