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Product Info Summary
| SKU: | A06825-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MAD2L1BP Antibody Picoband®
View all MAD2L1-binding protein Antibodies
SKU/Catalog Number
A06825-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAD2L1BP Antibody Picoband® catalog # A06825-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MAD2L1BP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06825-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MAD2L1BP recombinant protein (Position: Y20-E274).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06825-2 is reactive to MAD2L1BP in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
104553 MW
Background of MAD2L1-binding protein
MAD2L1-binding protein is a protein that in humans is encoded by the MAD2L1BP gene. The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06825-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A431 whole cell, human K562 whole cell, human Hela whole cell, human THP-1 whole cell, human HepG2 whole cell, human HEL whole cell, human 293T whole cell, human SH-SY5Y whole cell
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAD2L1BP using anti-MAD2L1BP antibody (A06825-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human HEL whole cell lysates,
Lane 7: human 293T whole cell lysates,
Lane 8: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD2L1BP antigen affinity purified polyclonal antibody (Catalog # A06825-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD2L1BP at approximately 37 kDa. The expected band size for MAD2L1BP is at 37 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-MAD2L1BP antibody (A06825-2).
Overlay histogram showing HEL cells stained with A06825-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAD2L1BP Antibody (A06825-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MAD2L1BP (Source: Uniprot.org, NCBI)
Gene Name
MAD2L1BP
Full Name
MAD2L1-binding protein
Weight
104553 MW
Superfamily
MAD2L1BP family
Alternative Names
Intraflagellar transport protein 88 homolog; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10; TPR repeat protein 10; IFT88; TG737; TTC10 MAD2L1BP CMT2 MAD2L1 binding protein MAD2L1-binding protein|caught by MAD2 protein|p31(comet)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAD2L1BP, check out the MAD2L1BP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAD2L1BP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAD2L1BP Antibody Picoband® (A06825-2)
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