Product Info Summary
SKU: | A08161 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-mAChR M4 (P277) Antibody
View all Muscarinic Acetylcholine Receptor M4/CHRM4 Antibodies
SKU/Catalog Number
A08161
Size
100ul
Form
Liquid
Description
Boster Bio Anti-mAChR M4 (P277) Antibody catalog # A08161. Tested in WB applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mAChR M4 (P277) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08161)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human CDC42EP3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08161 is reactive to CHRM4 in Human, Rat
Applications
A08161 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
53049 MW
Background of Muscarinic Acetylcholine Receptor M4/CHRM4
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts.
Joberty G., Mol. Cell. Biol. 19:6585-6597(1999).
Hirsch D.S., J. Biol. Chem. 276:875-883(2001).
Wiemann S., Genome Res. 11:422-435(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
![a08161 anti machr m4 p277 antibody wb testing 1 a08161 anti machr m4 p277 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a08161-anti-machr-m4-p277-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-mAChR M4 (P277) Antibody A08161
Western blot (WB) analysis of mAChR M4 (P277) pAb at 1:500 dilutionLane1:H1792 whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:K562 whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:SP2/0 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CHRM4 (Source: Uniprot.org, NCBI)
Gene Name
CHRM4
Full Name
Muscarinic acetylcholine receptor M4
Weight
53049 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
cholinergic receptor, muscarinic 4; CHRM4; HM4; M4; M4R; mAChR M4; muscarinic acetylcholine receptor M4 CHRM4 HM4, M4R cholinergic receptor muscarinic 4 muscarinic acetylcholine receptor M4|acetylcholine receptor, muscarinic 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHRM4, check out the CHRM4 Infographic
![CHRM4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHRM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-mAChR M4 (P277) Antibody (A08161)
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No publications found for A08161
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