Product Info Summary
SKU: | A07249-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-LYPLA1 Antibody Picoband®
SKU/Catalog Number
A07249-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LYPLA1 Antibody Picoband® catalog # A07249-2. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LYPLA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07249-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Standard Protein
E.coli-derived human BCL3 recombinant protein (Position: E118-A366). Human BCL3 shares 86.7% amino acid (aa) sequence identity with mouse BCL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07249-2 is reactive to LYPLA1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
52588 MW
Background of LYPLA1
Acyl-protein thioesterase 1 is an enzyme that in humans is encoded by the LYPLA1 gene. This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07249-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human K562 whole cell, human HEL whole cell, human RT4 whole cell, rat liver tissue, mouse liver tissue
IHC: human esophageal squamous carcinoma tissue, human rectum adenocarcinoma tissue, human lung cancer tissue
FCM: HEL cell
Validation Images & Assay Conditions
![a07249 2 lypla1 primary antibodies wb testing 1 a07249 2 lypla1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a07249-2-lypla1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of LYPLA1 using anti-LYPLA1 antibody (A16132-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA1 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA1 at approximately 25 kDa. The expected band size for LYPLA1 is at 25 kDa.
![a07249 2 lypla1 primary antibodies ihc testing 2 a07249 2 lypla1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a07249-2-lypla1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of LYPLA1 using anti-LYPLA1 antibody (A07249-2).
LYPLA1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LYPLA1 Antibody (A07249-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07249 2 lypla1 primary antibodies ihc testing 3 a07249 2 lypla1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a07249-2-lypla1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of LYPLA1 using anti-LYPLA1 antibody (A07249-2).
LYPLA1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LYPLA1 Antibody (A07249-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07249 2 lypla1 primary antibodies ihc testing 4 a07249 2 lypla1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a07249-2-lypla1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of LYPLA1 using anti-LYPLA1 antibody (A07249-2).
LYPLA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LYPLA1 Antibody (A07249-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07249 2 lypla1 primary antibodies fcm testing 5 a07249 2 lypla1 primary antibodies fcm testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a07249-2-lypla1-primary-antibodies-fcm-testing-5.png)
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Figure 5. Flow Cytometry analysis of HEL cells using anti-LYPLA1 antibody (A07249-2).
Overlay histogram showing HEL cells stained with A07249-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LYPLA1 Antibody (A07249-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LYPLA1 (Source: Uniprot.org, NCBI)
Gene Name
LYPLA1
Full Name
Acyl-protein thioesterase 1
Weight
52588 MW
Superfamily
AB hydrolase superfamily
Alternative Names
acyl-protein thioesterase-1; APT1; APT-1; EC 3.1.2; EC 3.1.2.-; hAPT1; LPL1acyl-protein thioesterase 1; LPL-I; Lysophospholipase 1; lysophospholipase ILYSOPLA; lysophospholipid-specific lysophospholipase; LysoPLA I LYPLA1 APT-1, APT1, LPL-I, LPL1, hAPT1 lysophospholipase 1 acyl-protein thioesterase 1|lysoPLA I|lysophospholipase I|lysophospholipid-specific lysophospholipase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LYPLA1, check out the LYPLA1 Infographic
![LYPLA1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LYPLA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LYPLA1 Antibody Picoband® (A07249-2)
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