Anti-LXR alpha/NR1H3 Antibody Picoband®

Product Name

Anti-LXR alpha/NR1H3 Antibody Picoband®

View all NR1H3 Antibodies

SKU/Catalog Number

PA1246-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LXR alpha/NR1H3 Antibody catalog # PA1246-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LXR alpha/NR1H3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1246-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LXR alpha, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1246-1 is reactive to NR1H3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

51 kDa

Calculated molecular weight

50396 MW

Background of NR1H3

LXRA is a tissue-specific cofactor that permits RXRA to function as a potent 9cRA receptor with a distinct target gene specificity. It specifically interacts with RXRA in vivo to form a functional heterodimer in which RXRA is the ligand-binding subunit. Additionally, LXR activity is critical for physiologic lipid metabolism and transport. LXRs are endogenous inhibitors of atherogenesis and are targets for therapeutic intervention in cardiovascular disease. Furthermore, LXRs and their ligands are negative regulators of macrophage inflammatory gene expression. LXR is also found that as a transcriptional switch that integrates hepatic glucose metabolism and fatty acid synthesis. The LXR-IDOL-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1246-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Liver Tissue, Rat Lung Tissue, Rat Spleen Tissue, Rat Kidney Tissue, Human Placenta Tissue

Validation Images & Assay Conditions

Gene/Protein Information For NR1H3 (Source: Uniprot.org, NCBI)

Alternative Names

Oxysterols receptor LXR-alpha;Liver X receptor alpha;Nuclear receptor subfamily 1 group H member 3;NR1H3;LXRA; NR1H3 LXR-a, LXRA, RLD-1 nuclear receptor subfamily 1 group H member 3 oxysterols receptor LXR-alpha|liver X nuclear receptor alpha variant 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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